Scanning spectrophotometer for high throughput fluorescence...

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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C250S458100, C356S318000

Reexamination Certificate

active

07411673

ABSTRACT:
A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.

REFERENCES:
patent: 4099872 (1978-07-01), White
patent: 4355871 (1982-10-01), Nevyas et al.
patent: 4657390 (1987-04-01), Doyle
patent: RE32598 (1988-02-01), White

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