Scanning fluorescent microscope

Radiant energy – Luminophor irradiation

Reexamination Certificate

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C250S461100

Reexamination Certificate

active

07365344

ABSTRACT:
A scanning fluorescent microscope includes a light source which irradiates a sample with an exciting light pulse, and a splitter which splits fluorescent photons emitted from the sample excited by the exciting light pulse, in at least first and second groups. The scanning fluorescent microscope also includes first and second detectors which detect the fluorescent photons of the first and second group, respectively, first and second counters which count numbers of fluorescent photons detected by the detectors, respectively, first and second correcting units which perform a predetermined correction to the numbers of fluorescent photons, an adder adding the numbers of fluorescent photons corrected, and an operating unit which calculates a florescent lifetime based on resulting numbers from the adder.

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