Scanning confocal microscope using fluorescence detection

Radiant energy – Photocells; circuits and apparatus – Optical or pre-photocell system

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359368, 356318, H01J 314

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active

052967034

ABSTRACT:
A scanning confocal microscopes scans a sample with an incident beam of ultraviolet radiation, in a raster scan pattern, causing the sample to fluoresce and emit visible radiation, a portion of which retraces a 5 portion of the path optical of incident beam, to a dichroic mirror that separates it from the incident beam for detection by a photomultiplier tube. A clock signal for clocking the output of the photomultiplier tube is provided by a reference beam system that directs a reference beam onto the same scanning mirror as is the incident beam, at a coincident location on that mirror, and from there through a Ronchi grating having a uniform series of alternating transparent and opaque regions. The resulting pulsed reference beam is detected by a second photomultiplier tube, to produce a clock signal that is an accurate representation of the instantaneous scan rate of the reference beam and, thereby, the scanning incident beam.

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