Scaffold for culturing cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...

Reexamination Certificate

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C435S180000, C435S395000

Reexamination Certificate

active

07435594

ABSTRACT:
A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised of polyacrylic acid, while taking advantage of synthetic polymers with low manufacturing cost, easy quality control and no risk of virus infection in cultured cell. Used as the cell culture scaffold is a gel containing asynthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group such as p-styrenesulfonic acid sodium salt (NaSS) and 2-acrylamide-2-methylpropane sulfonic acid sodium salt (NaAMPS).

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patent: 6617152 (2003-09-01), Bryhan et al.
patent: 6855746 (2005-02-01), Yoshitake et al.
patent: 7022523 (2006-04-01), Tsuzuki et al.
patent: 7078458 (2006-07-01), Irie et al.
Chen, Y., et al., “Endothelial Cell Culture on Structure Controlled Polacrylic Acid Hydrogel: Tailored for Application in Artificial Blood Vessel”,52ndSymposium of the Society of Polymer Science, Japan, 2 pages, (Sep. 2003).
Shiraishi, N., “Cultivation of Endothelial Cells on Synthetic Polymer Gels”,Summaries of Master Thesis Presentation of the Year Heisei 15(2003), pp. 2-5, (2003). English translation of Japanese document. With Declaration and English translation of Declaration.

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