Satellite RNA from bamboo mosaic virus as a vector for foreign g

Details Satellite RNA from bamboo mosaic virus as a vector for foreign g Satellite RNA from bamboo mosaic virus as a vector for foreign g

1995-07-28

1998-12-15

Degen, Nancy

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4353201, 435 5, C12N 1511, C12N 1563, C12N 1566, C12Q 170

Patent

active

058498915

ABSTRACT:
A satellite RNA was found to be naturally associated with bamboo mosaic virus (BaMV-V) isolated from infected Bambusa vulgaris McClure. Nucleotide sequence revealed that this satellite RNA genome contains 836 nucleotides and encodes a 20 kDa protein. Infectious transcripts have been generated from full length cDNA downstream T7 RNA polymerase promoter. Precise replacement of open reading frame (ORF) of cDNA of satellite RNA with sequence encoding bacterial CAT (chloramphenicol acetyltransferase) resulted in high level expression of CAT in infected dicotyledon plants, Chenopodium quinoa and tobacco (Nicotiana benthamiana) in the presence of baMV genomic RNA. Thus, this satellite system is potentially useful as a satellite-based plant expression vector.

REFERENCES:
Masuta, et al., GenBank accession No. M17182, 1987.

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