Sandwich immunoassay using piezoelectric oscillator

Details Sandwich immunoassay using piezoelectric oscillator Sandwich immunoassay using piezoelectric oscillator

1980-07-28

1982-02-09

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

23915, 310312, 324 71R, 422 61, 424 12, G01N 3354, H01L 4100

Patent

active

043148212

ABSTRACT:
A method and kit for determining the total amount of an immunologically-reactive substance in a liquid sample containing interfering material capable of binding to an antigen. The method involves the steps of: (1) contacting a liquid sample containing or suspected of containing an antibody with the surface of a piezoelectric oscillator having a layer of antigen specific for the antibody attached thereto; (2) washing and drying the oscillator; (3) measuring the resonance frequency of the oscillator; (4) contacting said surface of the oscillator with a liquid reagent containing an excess amount of a substance specifically reactive with all of the antibody bound to the oscillator in step (1); (5) washing and drying the oscillator; and (6) measuring the change in resonance frequency of the oscillator form the first measurement whereby the amount of total antibody bound to the oscillator in step (1) is distinguished from the interfering material bound in step (1).

REFERENCES:
patent: 4236893 (1980-12-01), Rice

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