Sandwich immunoassay kits for detecting ciguatoxin CTX3C

Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,... – Monoclonal antibody or fragment thereof

Reexamination Certificate

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C424S130100, C424S137100, C424S156100, C424S178100, C424S236100, C436S085000, C436S091000, C436S513000, C436S543000, C436S548000, C530S387100, C530S388100, C530S388200, C530S389800, C435S283100, C435S320100, C435S325000, C435S326000, C435S332000

Reexamination Certificate

active

07399470

ABSTRACT:
A sandwich immunoassay kit for detecting ciguatoxins based on a combination of two anti-ciguatoxin CTX3C monoclonal antibodies produced by hybridomas, 3D11 (deposited at IPOD, AIST under accession number FMRM PB-8293) and 10C9 (FMRM PB-8292). In particular, one of the antibodies is labeled and each of them binds specifically to a different site of ciguatoxin CTX3C.

REFERENCES:
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patent: 08-201392 (1996-08-01), None
patent: WO 03/016353 (2003-02-01), None
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Nagumo et al., Concise Synthesis of Ciguatoxin ABC-Ring Fragments . . . , Bioorganic & Medicinal Chemistry Letters, 2001, vol. 11, No. 15, pp. 2037-2040.
Sasaki et al., Stereocontrolled Synthesis of the JKLM Ring Fragment of Ciguatoxin, Journal of Organic Chemistry, 1999, vol. 64, No. 26, pp. 9399-9415.
Naar et al., Polyclonal and monoclonal antibodies to PbTx-2-type brevetoxins using minute amount of hapten-protein conjugates . . . , Toxicon, 2001, vol. 39, No. 6, pp. 869-878, Abs.Only.
Pauillac et al., Characterization of mice antisera elicited with a ciguatoxin tetracyclic synthetic ring fragment (JKLM) . . . , Toxicon, 2000, vol. 38, No. 5, pp. 669-685, Abs Only.
Hokama et al., A radioimmunoassay for the detection of ciguatoxin, Toxicon, 1977, vol. 15, No. 4, pp. 317-325, Abs Only.
Hokama et al., Cross-reactivity of highly purified okadaic acid (OA) . . . , Journal of Clinical Laboratory Analysis, 1992, vol. 6, No. 1, pp. 54-58, Abs Only.

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