Sandwich immunoassay and compositions for use therein

Details Sandwich immunoassay and compositions for use therein Sandwich immunoassay and compositions for use therein

1980-05-22

1982-11-30

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

23230B, 424 8, 424 12, C12Q 166

Patent

active

043616470

ABSTRACT:
An improved sandwich immunoassay for Toxoplasma gondii antigens associated with acute forms of Toxoplasma infection is disclosed. The assay employs either an F(ab').sub.2 fragment of IgG which reacts specifically with such antigens or a monoclonal antibody for such antigens as the substrate layer in the sandwich. The assay is sensitive and, unlike prior sandwich assays for the antigen, is not affected significantly by cross-reactions with rheumatoid factor.

REFERENCES:
patent: 4200436 (1980-04-01), Mochida et al.
patent: 4235869 (1980-11-01), Schwarzberg
Noel R. Rose et al., Editors, Principles of Immunology, Second Edition, MacMillan Publishing Co., pp. 49-52, and 513-528.

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