Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1984-05-02
1987-02-10
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 21, 436531, 436532, 436820, G01N 3353, G01N 33535, G01N 33545, G01N 33576
Patent
active
046422854
ABSTRACT:
A sensitive direct immunoassay system is provided for the detection of an antigen in body fluids. A single antibody which reacts with an antigen or antigens and which is bonded to an insoluble member, is incubated with a test sample. During this first period of incubation a portion of an antigen present in the test sample will combine with the antibody immobilized on the insoluble member. The antibody bonded member, to which antigen is attached, is then washed and incubated with an enzyme tagged antibody reagent. During the second incubation, the tagged antibody reacts with antigen fixed to the antibody member in the first incubation. Thus, an immobilized "sandwich" is formed of an insoluble member- antibody-antigen-enzyme tagged antibody. After the second incubation, the member is washed again to remove unreacted enzyme antibody reagent. The member is then exposed to a substrate which is converted by the enzyme to produce an end product. The tagged antibody reagent will be fixed in the second incubation only if antigen was present in the sample. The amount of enzyme tagged antitbody fixed is proportional to the amount of antigen or antigens present in the test sample up to the maximum capacity of the test. The concentration of the end product, and hence the amount of antigen or antigens, is determined by a spectrophotometer which measures the optical absorption of light by the end product. This readout is then compared against a standard value for both antigen negative and antigen positive samples.
REFERENCES:
patent: Re29169 (1977-04-01), Schuurs et al.
patent: 3646346 (1972-02-01), Catt
patent: 3654090 (1972-04-01), Schuurs et al.
patent: 3791932 (1974-02-01), Schuurs et al.
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3839153 (1974-10-01), Schuurs et al.
patent: 3852157 (1974-12-01), Rubenstein et al.
patent: 3867517 (1975-02-01), Ling
patent: 3876504 (1975-04-01), Koffler
patent: 3896217 (1975-07-01), Johnson
patent: 3896218 (1975-07-01), Charm et al.
patent: 3905767 (1975-09-01), Morris et al.
patent: 4016043 (1977-04-01), Schuurs
patent: 4034072 (1977-07-01), Mjos et al.
patent: 4154795 (1979-05-01), Thorne
Bonacker & Hevey, "Enzyme Immunoassays", Chapter 12 of Serum Abnormalities-Diagnostic and Clinical Aspects, Ed., Ritzmamann & Daniels (1975) Little, Brown & Co.).
Belanger et al., Clin, Chim, Acta, vol. 48, pp. 15-17 (1973).
Brzosko, W. J. et al., The Lancet, 1058, May 16, 1970.
Catt et al., J. Lab. and Clin Med., vol. 70, pp. 820-822 (1967).
Engvall, E. et al., The Journal of Immunology, 109 (1), 129-135 (1972).
Engvall et al., Biochem, Biophys, Acta., vol. 251, pp. 427-434 (1971).
Engvall, E. & Perlmann, P., "Enzyme-linked Immunosorbent Assay ELISA", Protides of the Biological Fluids, proceedings of the 19th Colloquium, 1971, ed. H. Peters, pp. 553-556.
Haberman, Z., klin Chem u. klin, Biochem, 51-55, 8th year Ja. 1970.
Miles, L. E. M. & Hales, C. N. Nature, 219, 186-189, Jul. 13, 1968.
Ling et al., Science, vol. 180, pp. 203-205 (1973).
Ruitenberg, et al., "Serodiagnosis of Trichinella Spiralis Infections in Pigs by Enzyme-Linked Immunoabsorbent Assays", Bull. Wld. Health., Org., 1974, 51, pp. 108-109.
Salmon, S. E. et al., The Journal of Immunology, 103, 129-137, Jul. 1969.
Saunders et al., "Disease Screening with Enzyme-Labelled Antibodies", J. Info. Dis., vol. 129 pp. 362-364, (Mar. '84).
Schuurs et al., The American Journal of the Medical Sciences, 270(1), 173-177 (1975).
Stimson et al., "An immunoassay for a Pregnancy Associated-Macroglobulin Using Antibody-Enzyme Conjugates", FEBS Letters, vol. 47, No. 1, pp. 190-192, (Oct. 1974).
van Weeman and Schuurs, "Immunoassay Using Antibody Enzyme Conjugates", FEBS Letters, vol. 43, No. 2 (July 1974), pp. 215-218.
Engvall & Perlman, "Enzyme-linked Immunosorbent Assay (ELISA) Quantitative Assay of Immunoglobulin G", Immunochemistry, vol. 8 (1971), pp. 871-874.
Anken Milton
Halbert Seymour P.
Diamedix Corporation
Marantz Sidney
LandOfFree
Sandwich EIA for antigen does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Sandwich EIA for antigen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sandwich EIA for antigen will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2362913