Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2003-11-21
2008-11-04
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07445893
ABSTRACT:
The invention provides sampling methods that permit the quantitative analysis of nucleic acid amplification reactions. The methods disclosed permit quantitative expression analysis of multiple genes or transcription units, both in the same amplification reaction and in multiple amplification reactions. In one aspect, the methods disclosed include, briefly, dispensing or withdrawing an aliquot from a reaction mixture at plural stages of an amplification regimen, separating and detecting nucleic acids in the aliquot, determining the quantity of a plurality of separated nucleic acid species in the aliquot, and for each separated nucleic acid species from each stage, correlating the quantity of the species with the stage at which the aliquot comprising the species was dispensed, thereby generating a profile of the amplification.
REFERENCES:
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5691146 (1997-11-01), Mayrand
patent: 6005663 (1999-12-01), Waterhouse et al.
patent: 6054035 (2000-04-01), Kambara
patent: 6207031 (2001-03-01), Adourian et al.
patent: 6221600 (2001-04-01), MacLeod et al.
patent: 6228589 (2001-05-01), Brenner
patent: 6479235 (2002-11-01), Schumm et al.
patent: 6482615 (2002-11-01), Tal et al.
patent: 6495326 (2002-12-01), Kurane et al.
patent: 6531282 (2003-03-01), Dau et al.
patent: 2002/0028452 (2002-03-01), Wittwer et al.
patent: 2002/0146688 (2002-10-01), Masataka
patent: 2002/0160361 (2002-10-01), Loehrlein et al.
patent: WO98/33942 (1998-08-01), None
patent: WO00/66995 (2000-11-01), None
patent: WO02/16652 (2002-02-01), None
patent: 2003035841 (2003-05-01), None
patent: WO03/035841 (2003-05-01), None
patent: 2004048528 (2004-06-01), None
Sanchez-Vega et al., Laboratory Investigation 82(1), 345A (Jan. 2002).
Sanchez-Vega et al., J. Mol. Diagnostics 4(4), 223-229 (Nov. 2002).
Li et al., Anal. Bioanal. Chem. 374, 269-273 (Aug.-Sep. 2002).
International Search Report of International Application No. PCT/US03/37420.
Holland, et al., “Detection of specific polymerase chain reaction product by utilizing the 5′→3′ exonuclease activity ofThermus aquaticusDNA polymerase”, (1991),Proc. Natl. Acad. Sci. USA, 88:7276-7280.
Borson, et al., “Direct Quantitation of RNA Transcripts by Competitive Single-Tube RT-PCR and Capillary Electrophoresis”, (1998),Biotechniques,25:130-137.
Rajcevic, et al., “Assessment of differential expression of oncogenes in gastric adenocarcinoma by fluorescent multiplex RT-PCR assay”, (2001),Pflugers Arch., Eur. J. Physiol., 442 (6 Suppl 1):R190-192.
Odin et al., “Rapid method for relative gene expression determination in human tissues using automated capillary gel electrophoresis and multicolor detection”, (1999),J. Chromatogr. B, 734:47-53.
George, et al., “Capillary electrophoresis methodology for identification of cancer related gene expression patterns of fluorescent differential display polymerase chain reaction”, (1997),J. Chromatogr. B., 695:93-102.
Omori, et al., “Comparative PCR: A Simple and Sensitive Method for Quantifying Low-Abundance mRNA Species”, (2000),Genomics,67:140-145.
Wiesner, Rudolf J., “Direct Quantification of Picomolar Concentrations of mRNAs by Mathematical Analysis of a Reverse Transcription/Exponential Polymerase Chain Reaction Assay,” Nucleic Acids Research, vol. 20, No. 21, 1992, pp. 5863-5864.
Wiesner, et al., “Counting Target Molecules by Exponential Polymerase Chain Reaction: Copy Number of Mitochondrial DNA in Rat Tissues,” Biochemical and Biophysical Research Communications, vol. 183, No. 2, 1992, pp. 553-559.
Meldrum Deirdre, “Automation for Genomics, Part One: Preparation for Sequencing,” Genome Research, vol. 10, No. 8, 2000, pp. 1081-1092.
Garcia, et al., “Scalable Transcriptional Analysis Routine—Multiplexed Quantitative Real-Time Polymerase Chain Reaction Platform for Gene Expression Analysis and Molecular Diagnostics,” Journal of Molecular Diagnostics, vol. 7, No. 4, Oct. 2005, pp. 444-454.
European Search Report, 03783738.2, Jan. 30, 2006.
Belgrader, P. and Marino, M.A., LRA, 9:3-7 (1997). “Automated Sample Processing Using Robotics for Genetic Typing of Short Tandem Repeat Polymorphisms by Capillary Electrophoresis.”
FitzGerald Mark J.
Horlick Kenneth R.
Nixon & Peabody LLP
Primera Biosystems, Inc.
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