Sample volume control for lateral flow chromatography

Details Sample volume control for lateral flow chromatography Sample volume control for lateral flow chromatography

1997-05-28

1999-12-28

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

422 55, 422 56, 422 57, 422 58, 4352871, 4352872, 4352877, 4352879, 435805, 435810, 435970, 436518, 436530, 436169, 436805, 436810, G01N 33558

Patent

active

060080566

ABSTRACT:
A method of assaying a preselected volume of sample on an chromatographic strip includes providing a sample receiving reservoir. This reservoir can receive a preselected volume of sample or a preselected volume of sample and reagent. The sample receiving reservoir has an overflow outlet. A moat is communicated to the overflow outlet of the sample receiving reservoir for receiving the sample or sample and reagent when the sample receiving reservoir is full. The mixed sample or sample and reagent fill the sample receiving reservoir, and discharge the excess sample through the overflow outlet to the moat. The sample receiving reservoir having the preselected volume of sample or sample and reagent is discharged on the chromatographic strip for assay. Provision is made for adding a density adding solute which is neutral to the chromatographic strip, the density adding solute occupying the sample receiving reservoir. As a consequence, dense sample first occupies the sample receiving reservoir with less dense excess sample being rejected.

REFERENCES:
patent: 5384264 (1995-01-01), Chen et al.

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