Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-08-04
1996-09-10
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
055545033
ABSTRACT:
Methods for processing samples which may contain mycobacteria which are compatible with both conventional culturing techniques and nucleic acid analysis. It has been found that the harsh chemical treatment previously thought necessary to obtain efficient lysis can be eliminated without loss of lysis efficiency, thereby eliminating reagents which may inhibit subsequent nucleic acid-based reactions. Heat alone is sufficient to lyse mycobacteria, even in buffers which do not contain detergents, chelators, enzymes, etc. Residual reagents introduced by conventional sample processing methods for culture can be successfully removed from the processed pellet without significant loss of microorganisms by at least two washings with saline, water, or buffers compatible with nucleic acid analysis and appropriate centrifugation.
REFERENCES:
patent: 5168039 (1992-12-01), Crawford et al.
patent: 5270184 (1993-12-01), Walker et al.
patent: 5376527 (1994-12-01), Robson et al.
V. Sritharan and R. H. Barker "A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR" Mol. Cell. Probes 5:385-395 (1991).
A. H. J. Kolk, et al. "Detection of Mycobacterium tuberculosis in Clinincal Samples by Using Polymerase Chain Reaction and a Nonradioactive Detection System" J. Clin. Microbiol. 30:2567-2575 (1992).
R. M. Shawar, et al. "Detection of Mycobacterium tuberculosis in Clinical Samples by Two-Step Polymerase Chain Reaction and Nonisotopic Hybridization Methods" J. Clin. Microbiol. 31:61-65 (1993).
P. DelPortillo, et al. "Amplification of a Species-Specific DNA Fragment of Mycobacterium tuberculosis and Its Possible Use in Diagnosis" J. Clin. Microbiol. 29:2163-2168 (1991).
D. V. Cousins, et al. "Use of Polymerase Chain Reaction for Rapid Diagnosis of Tuberculosis" J. Clin. Microbiol. 30:255-258 (1992).
G. E. Buck, et al. "Rapid, Simple Method for Treating Clinical Specimens Containing Mycobacterium tuberculosis To Remove DNA for Polymerase Chain Reaction" J. Clin. Microbiol. 30:1331-1334 (1992).
T. Victor, et al. "Purification of Sputum Samples Through Sucrose Improves Detection of Mycobacterium tuberculosis by Polymerase Chain Reaction" J. Clin. Microbiol. 30:1514-1517 (1992).
P. Shankar, et al. "Rapid diagnosis of tuberculous meningitis by polymerase chain reaction" Lancet 337:5-7 (1991).
D. DeWit, et al. "Direct Detection of Mycobacterium tuberculosis in Clinical Specimens by DNA Amplification" 28:2437-2441 (1990).
C. Pierre, et al. "Use of Reamplification Protocol Improves Sensitivity of Detection of Mycobacterium tuberculosis in Clinical Samples by Amplification of DNA" J. Clin. Microbiol. 29:712-717 (1991).
D. Thierry, et al. "The detection of Mycobacterium tuberculosis in uncultured clinical specimens using the polymerase chain reaction and a non-radioactive DNA probe" Molec. Cell. Probes 6:181-191 (1992).
A. Brisson-Noel, et al. "Rapid Diagnosis of Tuberculosis by Amplification of Mycobacterial DNA in Clinical Samples" Lancet Nov. 4, 1989:1069-1071.
B. B. Plikaytis, et al. "Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis BCG by a polymerase chain reaction assay" Molec. Cell. Probes 5:215-219 (1991).
K. D. Eisenach, et al. "Detection of Mycobacterium tuberculosis in Sputum Samples Using a Polymerase Chain Reaction" Am. Rev. Respir. Dis. 144:1160-1163 (1991).
G. Rahav, et al. "Development of sensitive methods for the detection of mycobacteria by DNA probes" FEMS Microbiol. Lett. 72:29-34 (1990).
L. G. Wayne and W. M. Gross "Isolation of Deoxyribonucleic Acid from Mycobacteria" J. Bacteriol. 95:1481-1482 (1968).
E. B. Hill, et al. "Purification of Mycobacterial Deoxyribonucleic Acid" J. Bacteriol. 112:1033-1039 (1972).
I. Baess "Isolation and Purification of Deoxyribonucleic Acid from Mycobacteria" Acta Path. Microbiol. Scand. Sect. B, 82:780-784 (1974).
J. W. U. Fries, et al. "Detection of Untreated Mycobacteria by Using Polymerase Chain Reaction and Specific DNA Probes" J. Clin. Microbiol. 29:1744-1747 (1991).
U. Sjobring, et al. "Polymerase Chain Reaction for Detection of Mycobacterium tuberculosis" J. Clin. Microbiol. 28:2200-2204 (1990).
R. J. Patel, et al. "Sequence Analysis and Amplification by Polymerase Chain Reaction of a Cloned DNA Fragment for Identification of Mycobacterium tuberculosis" J. Clin. Microbiol. 28:513-518 (1990).
C. Hackel, et al. "Specific identification of Mycobacterium leprae by the polymerase chain reaction" Molec. Cell. Probes 4:205-210 (1990).
A. Brisson-Noel, et al. "Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation" Lancet 338:364-366 (1991).
K. D. Eisenach, et al. "Polymerase Chain Reaction Amplification of a Repetitive DNA Sequence Specific for Mycobacterium tuberculosis" J. Infect. Dis. 161:977-981 (1990).
K. D. Evans, et al. "Identification of Mycobacterium tuberculosis and Mycobacterium avium-M. intracellulare Directly from Primary BACTEC Cultures by Using Acridinium-Ester-Labeled DNA Probes" J. Clin. Microbiol. 30:2427-2431 (1992).
Kocagoz et al. J. Clin. Micro. 31(6):1435-1438 (1993).
Walker et al. Nuc. Acids Res. 20(7):1691-1696 (1992).
Walker et al. P.N.A.S. 89:392-396 (1992).
Dey Margaret S.
Down James A.
Howard Deborah R.
Keating William E.
Little Michael C.
Becton Dickinson and Company
Fugit Donna R.
Zitomer Stephanie W.
LandOfFree
Sample processing method for Mycobacterium tuberculosis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Sample processing method for Mycobacterium tuberculosis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sample processing method for Mycobacterium tuberculosis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1319773