Sample processing for nucleic acid amplification

Details Sample processing for nucleic acid amplification Sample processing for nucleic acid amplification

2009-02-27

2009-12-29

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S007100, C435S007200, C435S091200, C536S023100, C536S024300

Reexamination Certificate

active

07638307

ABSTRACT:
Methods are described for the processing of biological samples for direct use in nucleic acid amplification without extracting or isolating the nucleic acids from the sample. In preferred embodiments, the processed sample provides the nucleic acid template in PCR-based assays.

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Grossman et al., High density multiplex detection of nucleic acid sequences: Oligonucleotide ligation assay and sequence-coded separation. Nucleic Acids Research 22:4527-34, 1994.
Spitzer et al., Identification of a new cystic fibrosis transmembrane regulator mutation in a severly affected patient. Eur. Respir. J 19:374-6, 2002.

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