Sample preparation method for liquid chromatography

Chemistry: analytical and immunological testing – Food or dairy products

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73 611C, 2101982, 210260, 21032171, 21032172, 21032175, 210656, 210659, 422 70, 436 89, 436 98, 436161, 436178, 436179, G01N 128, G01N 3008, G01N 3014, G01N 3302

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active

048371572

ABSTRACT:
A plurality of analytes are analyzed by liquid chromatography in a liquid biological sample containing many components which can interfere with analysis by liquid chromatography. The sample, containing the analytes of interest, are passed to a first passage of a dialyzer which removes molecules in the sample which interfere with analysis. A diluent is passed through a second passage of the dialyzer in which the two liquids are separated from each other by a semipermeable membrane having holes large enough to permit the analytes of interest to pass into the diluent but holes small enough to prevent passage of sample components which interfere with analysis. The analyte-containing diluent is passed to a retaining element for temporarily retaining the analytes of interest by adsorption and/or partition. The analytes of interest are removed from the retaining element by a second diluent which is then passed through a chromatography column with a detector and the analytes are analyzed chromatographically. The portion of the sample containing the interfering molecules is discarded.

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