Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-09-20
2005-09-20
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S005000, C435S091200, C435S091100, C435S024000, C435S024000
Reexamination Certificate
active
06946252
ABSTRACT:
A sample preparation apparatus for DNA analysis comprises a holder for separating specific primers on the basis of size, color, weight, dimension, or degree of magnetization, the specific primers having base sequences complementary to a plurality of DNA fragments to be amplified via PCR, and the specific primers being capable of binding respectively to the DNA fragments; and a reaction-solution-holding plate having a concavity which accommodates one edge of the holder and a PCR solution containing a common primer capable of hybridizing with the base sequence of an oligonucleotide introduced into the 5′-end of each of the DNA fragments, and the DNA fragments. The PCR amplification of the DNA fragments is carried out by using the specific primers (immobilized on the surfaces of a plurality of mutually separable supports with respect to each DNA fragments) and the common primer (a mobile primer common to all DNA fragments) to produce PCR amplification products inside the corresponding portions of the holder. The DNA fragments are derived from a plurality of DNAs to be amplified by PCR under the same conditions at the same time to avoid undesired mutual interference among the primers, and the PCR products can be separated and recovered for each of the DNA fragments. The sample preparation method for DNA analysis comprises the relevant amplifying, separating and recovering steps as described above.
REFERENCES:
patent: 5843660 (1998-12-01), Schumm et al.
patent: 5910406 (1999-06-01), Minter
patent: 5925525 (1999-07-01), Fodor et al.
patent: 6054035 (2000-04-01), Kambara
patent: 6060240 (2000-05-01), Kamb
patent: 6268148 (2001-07-01), Barany et al.
patent: 6518027 (2003-02-01), Kambara
patent: 0 846 776 (1997-12-01), None
patent: WO 93/18177 (1993-03-01), None
patent: WO 98/29736 (1997-12-01), None
patent: WO 99/09217 (1998-08-01), None
patent: WO 99/24822 (1998-11-01), None
European Search Report, EP 00 11 2246. Search completion date Mar. 18, 2002.
U.S. Appl. No. 09/260,543, filed Mar. 2, 1999, DNA Probe Array.
Nucleic Acids Research, 1977, vol. 25, No. 22, pp. 4694-4696.
LandOfFree
Sample preparation method and a sample preparation apparatus... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Sample preparation method and a sample preparation apparatus..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sample preparation method and a sample preparation apparatus... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3402212