Sample-collecting and assay device for use in the detection of b

Details Sample-collecting and assay device for use in the detection of b Sample-collecting and assay device for use in the detection of b

1998-01-23

2000-03-28

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 14, 435 15, 435 26, 435 28, 435 25, C12Q 142, C12Q 154, C12Q 148, C12Q 126

Patent

active

060430478

ABSTRACT:
An assay device comprises a tube, a removable top part and a bottom part, wherein an elongate member with a swab at its distal end is mounted on the top part, the top part includes a compartment containing liquid and partly defined by a first frangible membrane that can be ruptured to release the liquid into the tube, and the bottom part contains a reagent and is partly defined by a second frangible membrane that can be ruptured on movement of the bottom part relative to the tube.
Reaction occurring in the bottom part can be observed through a window. This reaction may be of the type involving amplification of say, ATP, e.g. by providing AMP, glucose-6-phosphate (G6P), adenylate kinase, glucokinase and reagents that convert glucose (G) to give a color, whereby the following reactions occur

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Chittock et al. (1991) "Light Amplification by a Coupled Biological System: ATP, Firefly Luciferase and Recycling of ATP". Biochemical Society Transactions. 19(160S).

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