Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1991-04-08
1993-05-04
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, 435 30, 435 29, 435 31, 435 39, 435 40, 4352528, C12Q 110, C12Q 104, C12Q 124, C12Q 102, C12Q 122, C12Q 106, C12Q 108, C12N 120
Patent
active
052081508
ABSTRACT:
An improved plating media incorporates TERGITOL.RTM.4, an alkyl sodium sulfate biological detergent, in a Xylose-lysine agar base. The plating media may also include peptone and sulfapyridine to further inhibit Citrobacter growth. The media is highly preferential for Salmonella, giving a greater number of Salmonella positives, and reduced competitive organisms. When incorporated with drag swab methodology employing skim milk or evaporated milk as a holding media, which may advantageously include novobiocin to suppress unwanted bacterial growth, coupled with a tetrathionate-culturing broth or other selective enrichment broth, the plating media completes a highly sensitive assay for the detection of Salmonella, particularly keyed for poultry and livestock handling structures, and for veterinary and human clinical laboratory applications.
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Joseph Sammy
Mallinson Edward
Miller Russell
Tate Christopher
Nucker Christine M.
Preston David R.
The United States of America as represented by the Secretary of
The University of Maryland
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