Saliva assay technique for heavy metal

Radiant energy – Ionic separation or analysis – Methods

Reexamination Certificate

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C250S281000, C073S061410, C073S061420, C436S073000, C436S077000

Reexamination Certificate

active

08049165

ABSTRACT:
A method for determining heavy metal loading in a subject includes collecting a saliva sample from the subject containing a concentration of a heavy metal. The saliva sample is subjected to inductively coupled plasma mass spectrometry to yield a heavy metal loading measurement for the subject. The saliva sample is readily collected on a substrate absorbing a preselected amount of saliva such as filter paper. As the amount of saliva necessary to saturate a given volume of substrate is known, the volume of saliva within a substrate is also known. The resulting heavy metal loading measurement is readily correlated with a blood level for the heavy metal in the subject.

REFERENCES:
patent: 2005/0218319 (2005-10-01), Bandura et al.
patent: 2006/0018800 (2006-01-01), Slowey et al.
patent: 2006/0275801 (2006-12-01), Henkin
patent: 2007/0209950 (2007-09-01), Althaus et al.
Nriagu et al. “Lead Levels in Blood and Saliva in a Low-Income Population of Detroit, Michigan”, International Journal of Hygiene and Enviromental Health, vol. 209 (2006) pp. 109-121, available online Jan. 27, 2006.
Thaweboon et al. “Lead in Saliva and its Relationship to Blood in the Residents of Klity Village in Thailand”, Southeast Asian Journal of Tropical Medicine and Public Health, vol. 36 (2005) pp. 1576-1579.
Koh et al., “Can salivary lead be used for biological monitoring of lead exposed individuals?”, 2003, Occupational and Enviromental Medicine vol. 60 pp. 696-698.
Nriagu et al. “Lead levels in blood and saliva in a low-income population of Detroit, Michigan”, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1819402/, publsihed online Jan. 27, 2006.

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