Saliva assay method and device

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 4, 435 29, 435805, 435810, 436 63, 422 56, 422 58, 422 61, 422102, 128760, 128762, 128769, 128771, C12Q 140, G01N 2100, A61B 500

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active

057143412

ABSTRACT:
An improved method for determining the presence of an analyte in an oral fluid sample. A portion of the sample is mixed or contacted with a chromogenic substrate effective to produce a colored product upon reaction with .alpha.-amylase present in the sample. After a selected reaction time, the reaction mixture is inspected to determine the level of such colored product produced. Production of a level of colored product above a selected threshold within the reaction time indicates that an effective volume of undiluted oral fluid sample has been collected which is sufficient to allow detection of the analyte. A device for use in the method is also disclosed.

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