Safe and versatile cloning vector

Details Safe and versatile cloning vector Safe and versatile cloning vector

1991-12-26

1994-03-01

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

43525233, 43525234, 4351721, 4353201, 4351723, 536 231, 935 27, 935 29, C12N 1500, C12N 1511, C12N 1570, C12N 1574

Patent

active

052906914

ABSTRACT:
A safe and versatile recombinant DNA cloning vector having at least two functionally different replication origins selected independently from the group consisting of a replication origin functional in Bacillus subtilis, a replication origin functional in Escherichia coli and a replication origin functional in Pseudomonas aeruginosa, and at least one DNA segment which provides resistance against at least one antibiotic after introduction into susceptible host cells. The origin for replication origin functional in Bacillus subtilis can be a restriction fragment of a plasmid of Gram positive bacteria, and that Escherichia coli or Pseudomonas aeruginosa can be a restriction fragment of a plasmid of Gram negative bacteria, e.g. a restriction fragment of plasmids pMS 140-1 or pMS 71.

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Thomas C. M. 1982 "Maintenance of Broad Host Range Plasmid RK-2 Replicans in Pseudomonas-aeruginosa" Nature 298(5875) 674-676.
Smith C. A. et al 1985 "Comparison of the Nucleotide Sequences of the Vegetative Replication Origins of Broad Host Range In cP Plasmids R751 & RK2 Reveals Conserved Features" NAR 13:557.
Laporta, M. Z. et al. 1986 Plasmids Coding for Drug Resistance and Localized Adherence to HeLa Cells in E. coli Infect. Imm. 51(2): 715.
Ishiwa H. (1985) Newshuttle Vector for E. coli and B. subtilis III Jap. J. Geract 60(5) 485-498.
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