SAF polypeptide, gene coding therefor, SAF promoter and expressi

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 6, 435 712, 435 91, 435169, 4351721, 4351723, 4352523, 43525235, 4353201, 435886, 536 27, 530350, 935 6, 935 8, 935 9, 935 22, 935 29, 935 33, 935 38, 935 39, 935 41, 935 59, 935 60, 935 61, 935 66, 935 72, 935 75, C12N 121, C12N 1531, C12N 1563, C12N 1576

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051186179

ABSTRACT:
The cloning and characterization of a newly isolated gene, referred to as saf (secondary metabolism activation factor) is disclosed. This gene encodes a new amino acid polypeptide, referred to as the SAF polypeptide, which directly or indirectly modulates the expression of extracellular enzymes in Streptomyces. DNA units or fragments which encode the SAF polypeptides are also disclosed, as are vectors containing said DNA, host organisms transformed with such vectors, and processes for preparing extracellular enzymes or heterologous polypeptides by culturing such host organisms.

REFERENCES:
patent: 4745056 (1988-05-01), Guterman et al.
Horinouchi et al., 1983, J. Bacteriol., vol. 155: 1238-1248.

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