Saccharomyces cerevisiae yeast strain with functional...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

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C435S483000, C435S440000, C435S071100

Reexamination Certificate

active

07052900

ABSTRACT:
The invention relates to a strain of the yeastSaccharomyces cerevisiaewhich, owing to deletion of the genomic sequences, no longer synthesizes hexose transporters and, as a consequence, can no longer grow on substrates with hexoses as the only carbon source, and whose ability of growing on a substrate with a hexose as the only carbon source is restored when it expresses a GLUT4 gene.

REFERENCES:
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R. Wieczorke et al., Concurrent knock-out of at least 20 transporter genes is required to block update of hexoses inSaccharomyces cerevisiae, FEBS Lett. vol. 464, (1999), pp. 123-128.
Kruckberg et al., The HXT2 Gene ofSaccharomyces cerevisiaeIs Required for High-Affinity Glucose Transport, Molecular and Cellular Biology, vol. 10, No. 11, Nov. 1990, pp. 5903-5913.
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Kasahara et al., Studies on the Glut Family Transporters: Use of the Yeast Expression System, International Symposium Kyushu Univ., 1997, pp. 201-212.
Kasahara et al., Tryptophan 388 in Putative Transmembrane Segment 10 of the Rat Glucose Transpoter Glut1 Is Essential for Glucose Transport, Journal of Biological Chemistry, vol. 273, No. 44, Oct. 30, 1998, pp. 29113-29117.
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