Saccharomyces cerevisiae strain productive of a heterologous pro

Details Saccharomyces cerevisiae strain productive of a heterologous pro Saccharomyces cerevisiae strain productive of a heterologous pro

1990-03-29

1992-11-10

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 692, 435255, 4353201, 4351723, C12N 119, C12P 2100

Patent

active

051622084

ABSTRACT:
The invention relates to a new strain of Saccharomyces cerevisiae productive of a heterologous protein, and more especially of hirudin, characterized by a suppression of the proteolytic function encoded by the PRC1 gene. More specifically, this deficiency is due to a mutation or more especially to a deletion of the PRC1 structural gene.

REFERENCES:
Wolf et al. Eur J Biochem 73:553 (1977) abstract only.
Zubenko et al. Genetics 102:679 (1982).
Valls et al. Cell 48:887 (1987).
Ma et al. Gene 58:201 (1987).
Struhl et al. PNAS 6:1035 (1979).
Lee et al. Biochem Biophy Res Comm 85:1135 (1978).

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