Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1990-08-13
1993-11-09
Rollins, John W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 536 2532, 536 2403, C07H 1700, C12Q 168
Patent
active
052604330
ABSTRACT:
The present invention provides nucleotides and polynucleotides which are chemically modified or labeled so as to be capable of ready detection when attached to and/or incorporated in nucleic acid material. More particularly, this invention provides a nucleotide selected from the group consisting of (i) a ribonucleotide having the formula, ##STR1## wherein PM is a phosphate moiety, SM is a sugar moiety, BASE is a pyrimidine, purine or 7-deazapurine moiety, and Sig is a saccharide moiety. PM is attached at the 2',3' or 5' position of SM, BASE is attached to the 1' position of SM from the N.sup.1 position when BASE is a pyrimidine or covalently attached to SM; and (ii) a nucleotide having the formula, ##STR2## wherein PM, SM, BASE and Sig are as defined above but PM is attached to the 3' or the 5' position of SM when said nucleotide is a deoxyribonucleotide and at the 2', 3' or 5' position when said nucleotide is a ribonucleotide, BASE is attached to the 1' position of SM from the N.sup.1 position when BASE is a pyrimidine or the N.sup.9 position when BASE is a purine, and wherein Sig is covalently attached to PM.
REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
Engelhardt Dean
Kirtikar Dollie
Kline Stanley
Rabbani Elazar
Stavrianopoulos Jannis G.
Enzo Diagnostics, Inc.
Fedus Ronald C.
Rollins John W.
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