Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-03-07
2010-10-19
Jiang, Shaojia Anna (Department: 1623)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
Reexamination Certificate
active
07816517
ABSTRACT:
In one embodiment of the present invention, a first test tube including therein a dehydrated sample containing a sugar chain and a labeling agent solution made of 2-aminopyridine/acetic acid are stored in a well-closed container with the first test tube and the labeling agent solution secluded from each other. The labeling agent solution is heated, and the 2-aminopyridine is evaporated so as to be in contact with the sugar chain, thereby obtaining a pyridylamination sugar chain from the sugar chain. As a result, it is possible to provide the saccharide fluorescence labeling apparatus and the saccharide fluorescence labeling method whereby it is possible to simplify and ensure an operation for reacting saccharide into the pyridylamination sugar chain.
REFERENCES:
patent: 4975533 (1990-12-01), Kondo et al.
A. Kondo et al. “Improved Method for Fluorescence Labeling of Sugar Chains with Sialic Acid Residues”. Agricultural and Biological Chemistry, vol. 54, pp. 2169-2170. Aug. 1990.
S. Hase et al. “Tagging of Sugars with Fluorescent Compound, 2-Aminopyridine”. The Journal of Biochemistry, vol. 85, No. 1 pp. 217-220. Jan. 1979.
A. K. Vrkic et al. “Using Non-Covalent Complexes to Direct the Fragmentation of Glycosidic Bonds in the Gas Phase”. Jouranl of American Society for Mass Spectrometry, vol. 15, pp. 715-724. 2004.
Shin-ichi Nakakita et al. “Gas-Phase Pyridylamination of Saccharides: Development and Applications”. Glycoconjugate Journal vol. 24, No. 6/7, p. 367. Oct. 2007.
Shin-ichi Nakakita et al. “Gas-Phase Pyridylamination of Saccharides: Development and Applications”. Analytical Chemistry vol. 79., No. 7, pp. 2674-2679. Apr. 2007.
Shin-ichi Nakakita et al., “Gas-Phase Pyridylamination of Saccharides: Development and Applications”.Analytical Chemistry, vol. 79, No. 7, pp. 2674-2679 (Apr. 1, 2007).
Shin-ichi Nakakita et al. “Gas-Phase Pyridylamination of Saccharides: Development and Applications”. Analytical Chemistry vol. 24, No. 6/7, p. 367. Oct. 2007.
Hase Sumihiro
Nakakita Shin-ichi
Harness & Dickey & Pierce P.L.C.
Jiang Shaojia Anna
Krishnan Ganapathy
National University Coporation Kagawa University
Osaka University
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