Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-06-24
1998-10-13
McElwain, Elizabeth
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 6, 4353201, 536 232, 536 236, 536 243, 536 2433, 800205, A01H 500, C12N 1529, C12N 1582
Patent
active
058210946
ABSTRACT:
The S-locus of Brassica contains the genetic information that encodes for self-incompatibility. In its first aspect, it is directed to an isolated gene, the SRK-910 gene, that segregates with the self-incompatibility phenotype. In its second aspect, the present invention is directed to an isolated cDNA that corresponds to the isolated gene and that has 2749 nucleotides.
The isolated cDNA of the present invention encodes for a protein, i.e., the S-locus receptor kinase-910 protein ("the SRK-910 protein") which is also a part of the present invention. The SRK-910 protein, has 858 amino acids and is encoded for by the first 2575 nucleotides of the isolated cDNA of the present invention.
The present invention is also directed to an oligonucleotide probe that is capable of distinguishing the SRK-910 gene from partially homologous genes at the S-locus that encode for the S-locus glycoproteins.
The present invention is also directed to a transfer vector comprising the cDNA for the SLG-910 allele in combination with the cDNA of claim 1.
Finally, the present invention is also directed to a method for conferring the self-incompatible phenotype on a self-compatible plant comprising transferring the disclosed transfer vector into a plant that is capable of assimilating the transfer vector and expressing self-incompatibility.
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Goring Daphne R.
Rothstein Steven J.
McElwain Elizabeth
University of Guelph
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