S-adenosylmethionine synthetase mutants, the DNAs encoding...

Details S-adenosylmethionine synthetase mutants, the DNAs encoding... S-adenosylmethionine synthetase mutants, the DNAs encoding...

2007-08-30

2010-12-28

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S088000, C435S015000

Reexamination Certificate

active

07858351

ABSTRACT:
The invention discloses a series ofMethanococcus jannaschiiS-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.

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