Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-09-26
1999-08-24
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 911, 4352523, 4352525, 4353201, 536 231, 536 2431, C12N 1500, C12N 120, C12N 1563, C07H 2104
Patent
active
059424192
ABSTRACT:
RsbW-1 polypeptides and DNA (RNA) encoding such RsbW-1 and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such RsbW-1 for the treatment of infection, particularly bacterial infections. Antagonists against such RsbW-1 and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of RsbW-1 nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding SigB operon and for detecting the polypeptide in a host.
REFERENCES:
Boylan, et al., "Activation of Bacillus subtilis Transcription Factor .sigma..sup.B by a Regulatory Pathway Responsive to Stationary-Phase Signals",; Journal of Bacteriology, vol. 174, No 11, pp. 3695-3706 (Jun. 1992).
Kullick, et al., "The alternative sigma factor .sigma..sup.B in Staphylococcus aureus: regulation of the sigB operon in response to growth phase and heat shock"; Arch Microbiol 167, pp. 151-159; Springer-Verlag (1997).
Voelker, et al., "The Yeast Two-Hybrid System Defects Interactions between Bacillus subtilis .sigma..sup.B Regulators"; Journal of Bacteriology; vol. 178, No. 23, pp. 7020-7023 (Dec. 1996).
Voelker, et al., "Reactivation of the Bacillus subtilis Anti-.sigma..sup.B Antagonist, RsbV, by Stress- or Starvation-Induced Phosphatase Activities"; Journal of Bacteriology, vol. 178, No. 18, pp. 5456-5463, (Sep. 1996).
Alper, et al., "Role of Adenosine Nucleotides in the Regulation of a Stress-response Transcription Factor in Bacillus subtilis"; J.Mol. Biol. 260, pp. 165-177 (1996).
Yang, et al., "Opposing pairs of serine protein kinase and phosphatases transmit signals of environmental stress to activate a bacterial transcription factor", Genes & Development 10, pp. 2265-2275 (1996).
Voelker, et al., "Seperate Mechanisms Activate .sigma..sup.B of Bacillus subtilis in Response to Environmental and Metabolic Stresses", Journal of Bacteriology, vol. 177, No. 13, pp. 3771-3780, (Jul. 1995).
Dufour, et al.,:"Interactions between a Bacillus subtilisAnti-.sigma. Factor (RsbW) and Its Antagonist (RsbV)", Journal of Bacteriology, vol. 176, No. 7, pp. 1813-1820 (Apr. 1994).
Beloin, et al., GenBank Submission, Accession No. AB001488 "Characterization of an Irp-like (IrpC) gene from Bacillus subtilis". Oct. 21, 1997.
Yang, et al., GenBank Submission, Accession No. AF035567 "Opposing Pairs of serine protein kinases and phosohatases transmit signals of environmental stress to activate a bacterial transcription factor", Dec. 4, 1997.
Kullik, et al., GenBank Submission, Accession No. Y07645 "The alternative sigma factor sB in Staphylococcus aurues: regulation of the sigB operon in response to growth phase and heat shock". Apr. 9, 1997.
Wu, et al., GenBank Submission, Accession No. Y09929 "Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: Molecular cloning and DNA sequencing". Dec. 12, 1996.
Wu et al., Sigma B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: molecular cloning and DNA Sequencing, J. Bacteriology, vol. 178(20), pp. 6036-6042, Oct. 17, 1996.
Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd edition. Cold Spring Harbor Press, 1989.
Boswell et al., Sequence comparison and alignment: the measurement and interpretation of sequence similarity, Computational Molecular Biology, pp. 161-178, 1988.
Fosberry Andrew P
Lawlor Elizabeth J
Nicholas Richard O
Degen Nancy
Deibert Thomas S.
Gimmi Edward R.
King William T.
SmithKline Beecham p.l.c.
LandOfFree
RsbW-1 encoding nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with RsbW-1 encoding nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and RsbW-1 encoding nucleic acids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-465685