Rotavirus enterotoxin NSP4 and methods of using same

Details Rotavirus enterotoxin NSP4 and methods of using same Rotavirus enterotoxin NSP4 and methods of using same

2000-11-03

2004-01-06

Housel, James (Department: 1648)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Virus or component thereof

C424S139100, C424S147100, C424S159100, C424S204100, C530S300000, C530S324000, C435S236000

Reexamination Certificate

active

06673355

ABSTRACT:

FIELD OF THE INVENTION
This invention relates to the viral enterotoxin NSP4 and to methods for using it, or antibodies/antisera thereto, as diagnostic agents, vaccines and therapeutic agents for the detection, prevention and/or treatment of rotaviral disease, for the prevention of stunted growth in animals and children caused by rotaviral infection and for the treatment of cystic fibrosis. This invention also relates to methods and animal models for 1) the screening for viral enterotoxins, 2) the detection of viral enterotoxins and 3) the identification of viral enterotoxins.
BACKGROUND OF THE INVENTION
Rotaviruses are the leading cause of severe, life-threatening viral gastroenteritis in infants and animals (Kapikian et al., 1996) and are associated with sporadic outbreaks of diarrhea in elderly (Halvorsrud 1980) and immunocompromised patients (Holzel et al., 1980). These viruses have a limited tissue tropism, with infection primarily being restricted to cells of the small intestine (Estes et al., 1994). Rotavirus infections also cause morbidity and mortality in many animal species. Moreover, the outcome of infection is age-related; although rotaviruses may infect individuals and animals of all ages, symptomatic infection (i.e., diarrhea) generally occurs in the young (6 months—2 years in children, and up to 14 days in mice), and the elderly.
Age-related host factors which may influence the outcome of infection have been proposed to include 1) differences in the presence/quantity of virus-binding receptors on mature villus epithelial cells, 2) virus strains with a specific spike protein (VP4), 3) passive immunity acquired by maternal antibody or in colostrum, and 4) reduced levels of proteases in the young.
Disease resulting from rotavirus infection in mice has been studied more extensively than in any other species and an age restriction of disease has been reported by several investigators (Ramig 1988; Wolf et al. 1981; Riepenhoff-Talty et al., 1982). Only mice less than 14 days of age develop diarrhea following oral inoculation of murine rotavirus, and the peak age at which animals are most likely to develop diarrhea (6-11 days) corresponds to the age when rotavirus can bind to mouse enterocytes (Riepenhoff-Talty et al., 1982). Treatment of 8 day old mice with cortisone acetate which promotes premature maturation of intestinal epithelial cells, results in a reduced susceptibility to rotavirus-induced diarrhea, although the mice can still be infected (Wolf et al., 1981). These data were interpreted to suggest that the capacity of murine rotaviruses to induce diarrhea in young, but not adult mice, is due to the quantity of rotavirus-binding receptors on the surface of villus epithelial cells in the young mouse intestine.
When compared to rotavirus infections in other species, rotavirus infections in mice show minimal histologic alterations. That is, villus blunting is limited and transient, and crypt cell hyperplasia is not present. In addition, the loss of villus tip epithelial cells is more limited in mice than in other animals. Instead, vacuolization of enterocytes on the villus tips is a predominant feature in symptomatic rotavirus infection in mice and virus replication may be abortive (Greenberg et al., 1981). The lack of extensive pathologic alterations in the mouse intestine during symptomatic infections has remained a puzzle; one interpretation of this phenomenon is that a previously unrecognized mechanism of diarrhea induction may be active in symptomatic rotavirus infection in mice.
Despite the prevalence of rotavirus infections and extensive studies in several animal models and many advances in understanding rotavirus immunity, epidemiology, replication and expression, rotavirus pathogenesis, specifically, the mechanism of diarrhea induction, remains poorly understood. Proposed pathophysiologic mechanisms by which rotaviruses induce diarrhea following viral replication and viral structural protein synthesis include malabsorption secondary to the destruction of enterocytes (Graham et al., 1984), disruption of transepithelial ion homeostasis resulting in fluid secretion (Collins et al., 1988), and local villus ischemia leading to vascular damage and diarrhea (Osborne et al., 1988). However, these proposed mechanisms do not explain cases of rotavirus-induced diarrhea observed prior to, or in the absence of, histopathologic changes (Theil et al., 1978; McAdaragh 1980; Saif et al., 1976).
On the other hand, the pathophysiology of bacterial-induced diarrhea based on interactions with intestinal receptors and bacterial enterotoxins is well understood (Burges et al., 1978; Gianella et al., 1981; Krause et al., 1990). The heat-stable toxin A and the heat-labile toxin of
E. coli
, and guanylin (an endogenous, 15 amino acid intestinal ligand originally isolated from rat jejunum) induce diarrhea by binding a specific intestinal receptor, increasing cAMP or cGMP, and activating a cyclic nucleotide signal transduction pathway (Giannella et al., 1993; Currie et al., 1992; Field et al., 1978; Forte et al., 1992). The net effect of these bacterial toxins is to increase Cl
−
secretion, and decrease Na
+
and water absorption.
Previous studies in insect cells indicated that a receptor-mediated phospholipase C pathway is associated with the increases in [Ca
2+
]
i
, following exogenous treatment of cells with NSP4 or NSP4 114-135 peptide (Tian et al., 1994). The rotavirus nonstructural ER glycoprotein, NSP4, has been shown to have multiple functions including the release of calcium from the endoplasmic reticulum (ER) in SF9 insect cells infected with recombinant baculovirus containing the NSP4 cDNA (Tian et al., 1994; Tian et al., 1995). In addition, NSP4 disrupts ER membranes and may play an important role in the removal of the transient envelope from budding particles during viral morphogenesis. NSP4 114-135, a 22 aa peptide of NSP4 protein, has been shown to be capable of mimicking properties associated with NSP4 including being able to (i) mobilize intracellular calcium levels in insect cells when expressed endogenously or added to cells exogenously (Tian et al., 1994; Tian et al., 1995), and (ii) destabilize liposomes.
Expression of NSP4 in insect cells increased [Ca
2+
]
i
levels from a subset of the thapsigargin-sensitive store (ER) (Tian et al., 1995). The [Ca
2
+]
i
mobilized by NSP4 or the NSP4 114-135 peptide added exogenously to cells was blocked by a phospholipase C inhibitor, the U-73122 compound, suggesting that a receptor-mediated pathway is responsible for the calcium release from the ER induced by NSP4 (Tian et al., 1995). The [Ca
2+
]
i
mobilized by NSP4 expressed intracellularly was not blocked by the U-73122 compound, suggesting that a second pathway is responsible for the calcium release from the ER induced by intracellular NSP4 (Tian et al., 1995).
SUMMARY OF THE INVENTION
The present invention discloses herein a method of immunization against rotavirus infection or disease comprising administering to a subject a peptide NSP4 112-175 or NSP4 112-150 or a toxoid thereof. Further, the present invention discloses a method of immunization against rotavirus infection or disease comprising administering to a subject a non-gylcosylated NSP4 protein or a toxoid of NSP4. The immunizations may result in both homotypic and heterotypic immunity.
In another specific embodiment, it is also provided a method of passive immunization against rotavirus infection comprising administering to an expectant mother a peptide NSP4 112-175, NSP4 112-150 or a toxoid thereof. Yet further, the present invention discloses a method of passive immunization against rotavirus infection comprising administering to an expectant mother a non-gylcosylated NSP4 protein or a toxoid of NSP4. The immunizations may result in both homotypic and heterotypic immunity.
A specific embodiment of the present invention is that the NSP4 peptide (e.g., NSP4 112-175 or NSP4 112-150) or toxoid is produced by a synthetic method. In a furth

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