Rotating potentiometric electrode

Details Rotating potentiometric electrode Rotating potentiometric electrode

2005-06-21

2005-06-21

Noguerola, Alex (Department: 1753)

Electrolysis: processes, compositions used therein, and methods

Electrolytic analysis or testing

For ion concentration

C204S418000

Reexamination Certificate

active

06908542

ABSTRACT:
A rotating electrode configuration lowers the detection limits of polyion-sensitive membrane electrodes. Planar potentiometric polycation and polyanion-sensitive membrane electrodes were prepared by incorporating tridodecylmethylammonium chloride and calcium dinonylnaphthalene sulfonate, respectively, into plasticized PVC or polyurethane membranes, and mounting discs of such films on an electrode body housed in a rotating disk electrode apparatus of the type used in voltammetry. Due to the unique non-equilibrium response mechanism of such sensors, rotation of the polyion-sensitive membrane electrodes at 5000 rpm resulted in an enhancement in the detection limits toward heparin (polyanion) and protamine (polycation) of at least 1 order of magnitude (to 0.01 U/ml for heparin; 0.02 μg/ml for protamine) over that observed when the EMF responses of the same electrodes were assessed using a stir-bar to achieve convective mass transport.

REFERENCES:
patent: 5236570 (1993-08-01), Ma et al.
patent: 5453171 (1995-09-01), Ma et al.
patent: 5607567 (1997-03-01), Yun et al.
Lukashev, “Polarizatio haracteristics of monopolar ionite membranes,” Russian Journal of Environmental Development, Moscow, Russia (2000), 36(4), 367-372, Apr. 2000.
Pages 16 and 25 of the Orion 1997 Laboratory products and Electrochemistry Handbook.
Ramamurthy, et al., “Determination of Low-Molecular-Weight Heparins and Their Binding to Protamine and a Protamine Analog Using Poly-Sensitive Membrance Electrodes”,Analytical Biochemistry, vol. 266, No. 1, Jan. 1, 1999, pp. 116-124.
Wingard, Lemuel B., et al., “Immobilized Glucose Oxidase in the Potentiometric Detection of Glucose”,Applied Biochemistry and Biotechnology, vol. 9, Feb. 1994, pp. 95-104.
Meyerhoff and Ye, “Rotating Electrode Potentionmetry: Lowering the Detection Limits of Nonequilibrium Polyion-Sensitive Membrane Electrodes”,Analytical Chemistry, vol. 73, pp. 332-336.
Fu, et al.,Response Mechanism of Polymer Membrane-Based Potentiometric Polyion Sensors, Anal. Chem., vol. 66, p. 2250-2259 (1994).
Mathison, et al.,Renewable ph Cross-Sensitive Potentiometric Heparin Sensors with Incorporated Electrically Charged H+ lonophores, Anal. Chem., vol. 71, pp. 4613-4621 (1999).

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