Rotating apparatus for isoelectric focusing

Details Rotating apparatus for isoelectric focusing Rotating apparatus for isoelectric focusing

1984-05-07

1986-05-13

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041832, 2041823, G01N 2728

Patent

active

045884927

ABSTRACT:
This disclosure is directed to an isoelectric focusing apparatus, wherein stabilization of the fluid containing the isolated proteins is achieved by carrying out the separation in a rotating cylinder with the separation cavity of the cylinder being segmented by means of filter elements. The filter elements are constituted of a material offering some degree of resistance to fluid convection, but allowing relatively free and unhindered passage of current and transport of proteins. The combined effect of segmentation and rotation has been found to be superior to either segmentation or rotation alone in maintaining the stability of the migrated fractions.

REFERENCES:
patent: 3844926 (1974-10-01), Smyth et al.
patent: 4040940 (1977-08-01), Bier
patent: 4177130 (1979-12-01), Herrmann et al.
patent: 4204929 (1980-05-01), Bier
patent: 4310408 (1982-01-01), Rose et al.
patent: 4362612 (1982-12-01), Bier
patent: 4465583 (1984-08-01), Lovegrove

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