Root-specific promoter

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 2371, 536 236, 4351723, 4352404, 4353201, 800205, 800DIG56, C12N 1529, C12N 1564, C12N 1582, C12N 1533

Patent

active

058378480

ABSTRACT:
A DNA which has the sequence shown in FIG. 5 and which defines a gene promoter region has been isolated from maize roots. The promoter may be used for driving expression of foreign genes in the roots of plants. This is particularly useful for expressing an insecticidal toxin, such as a delta-endotoxin of Bacillus thuringiensis, to impart resistance to insect attack on the roots of plants by Coleopteran insects.

REFERENCES:
patent: 4806669 (1989-02-01), Puskaric
Benfey, et al: "The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants", Science, vol. 250: pp. 959-966.
Potrykus: "Gene Transfer to Cereals: An Assessment", Bio/Technology, 8: pp. 535-542.
Koster-Topfer, et al: "A Class II patatin promoter is under developmental control in both transgenic potato and tobacco plants", Moi Gen Genet (1989) 219: pp. 390-396.
Barton, et al: "Bacillus thruingiensis .delta.-Endotoxin Expresses in Transgenic Nicotiana tabacum Provides Resistance to Lepidopteran Insects", Plant Physiol.(1987)85, pp. 1102-1109.
Montoliu, et al: A tandem of .alpha.-tubulin genes preferentially expressed in radicular tissues from Zea mays Plant Molecular Biology 14: 1-15, 1989.
Hillel et al: "An Octipine Synthase Enhancer Element Directs Tissue-Specific Expression and Binds ASF-1, a Factor from Tobacco Nuclear Extracts", The Plant Cell, vol. 1,977-1984, Oct. 1989-American Society of Plant Physiologists.
Gordon-Kamm, et al: "Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants", The Plant Cell. vol. 2, 603-618, Jul. 1990 American Society of Plant Physiologists.
John et al: "Isolation and Characterization of Root-Prefferential CDNA Clones from Zea mays", Abstracts, Journal of Cellular Biochemistry, Supplement 15A, 1991, Jan. 10 -24, 1991, p. 133.
Baynton et al: Isolation of DNA sequences determining organ enchanced abstracts, vol 41, May 1990.
Ludwig, et al: "A Regulatory Gene as a Novel Visible Marker for Maize Transformation" Jan. 1990, Science, vol 24, pp. 449 -450.
Kawamura et al: "Phosphoenolpyruvate Carboxylase Prevalent in Maize Roots: Isolation of a cDNA Clone and Its Use for Analyses of the Gene and Gene Expression", J. Biochem, vol. 107, No. 1, 1990 -pp. 165-168.
Kim et al. Plant Molecular Biology. 24:105-117, Jan. 1994.

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