Root-specific conifer gene promoter and its use

Details Root-specific conifer gene promoter and its use Root-specific conifer gene promoter and its use

2007-10-23

2007-10-23

Collins, Cynthia (Department: 1638)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100

Reexamination Certificate

active

10422714

ABSTRACT:
There is provided an isolated polynucleotide sequence useful as a promoter in plant root tissue. The sequence is derived from the promoter for the Western white pine gene PR10. The sequence comprises at least 45 nucleotidcs, and has at least two Dof elements which individually may be in a forward or inverted orientation. Each Dof element has a sequence NNNWAAAGNNN, wherein N is A, T, G, or, C, and, W is A or T. The Dof elements are separated from each other by between 10 and 20 nucleotides. The sequence also has a TATA box located between 15 and 25 nucleotides in the 3′ direction of the closest Dof element. In some instances, the polynucleotide has one Dof element with the sequence NNNWAAAGNNN and another with the sequence NNNCTTTWNNN. In some instances the polynucleotide sequence is one where the 5′ Dof element sequence is CTCTCTTTAAT, and another Dof element (which may be the next Dof element) sequence is TTTTAAAGGTT and the TATA box sequence is TACAATAAATA or TATAWAWA, wherein W is A or T. Also provided are sequences useful in modulating gene expression in stem and leaf tissue.

REFERENCES:
Kim Y. et al. (A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. Plant Mol Biol. Jan. 1994;24(1):105-17).
Yanagisawa S. Dof1 and Dof2 transcription factors are associated with expression of multiple genes involved in carbon metabolism in maize. Plant J. Feb. 2000;21(3):281-8.
Yanagisawa S. et al. Involvement of maize Dof zinc finger proteins in tissue-specific and light-regulated gene expression. Plant Cell. Jan. 1998;10(1):75-89.
Shen Q. et al. Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. Plant Cell. Jul. 1996;8(7):1107-19.
Curie C. et al. Modular organization and development activity of an Arabidopsis thaliana EF-1 alpha gene promoter. Mol Gen Genet. Apr. 1993;238(3):428-36.

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