Room temperature stable reagent semi-spheres

Details Room temperature stable reagent semi-spheres Room temperature stable reagent semi-spheres

1994-09-02

1996-10-15

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 24, 435 23, 435 18, 435 15, 435 16, 435 791, 435 681, 435183, 435174, 435178, 435179, 435188, 435810, 435975, 436 17, C12Q 100, C12Q 137, G01N 3353

Patent

active

055653189

ABSTRACT:
A reagent semi-sphere is disclosed comprising at least one biological reagent and a glass forming filler material in a concentration sufficient to facilitate formation of a glassy, porous composition, wherein the reagent semi-sphere is room temperature stable, water soluble, and has a T.sub.g above room temperature. A method is provided for making the reagent semi-sphere comprising providing an aqueous solution of a buffered biological reagent; mixing a glass forming filler material with the buffered reagent solution to form an emulsion wherein the concentration of the filler material is sufficient to facilitate formation of a glassy, porous composition having a predetermined semi-spherical shape; dispensing the emulsion in the form of substantially uniform droplets; collecting the droplets on an inert medium to form semi-spheres; and vacuum drying the droplets, under conditions suitable for maintaining the predetermined semi-spherical shape, to form the reagent semi-sphere; wherein the reagent semi-sphere is room temperature stable, water soluble, and has a T.sub.g above room temperature.

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