Rolling circle synthesis of oligonucleotides and amplification o

Details Rolling circle synthesis of oligonucleotides and amplification o Rolling circle synthesis of oligonucleotides and amplification o

1995-02-23

1998-02-03

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 435 911, 536 245, 536 243, 536 2432, 536 2433, 536 231, C12Q 168, C12Q 170, C12P 1934, C07H 2104

Patent

active

057143200

ABSTRACT:
The present invention provides methods for synthesis, selection, and amplification of DNA and RNA oligonucleotides and analogs. The method for synthesizing an oligonucleotide involves: providing an effective amount of an isolated circular oligonucleotide template which comprises at least one copy of the desired oligonucleotide sequence linked to a cleavage site; providing an effective amount of an isolated oligonucleotide primer; annealing the primer to the circular template to form a primed circular template; and combining the primed circular template with an effective amount of at least two types of nucleotide triphosphates and an effective amount of a polymerase enzyme to form a nucleotide multimer complementary to the circular oligonucleotide template, wherein the nucleotide multimer comprises multiple copies of the oligonucleotide sequence joined end to end. Preferably, the nucleotide multimer is cleaved to produce oligonucleotides having well-defined ends.

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