Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-07-22
2009-11-17
Myers, Carla (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S091500
Reexamination Certificate
active
07618776
ABSTRACT:
Disclosed are compositions and a method for of amplifying nucleic acid sequences useful for detecting the presence of molecules of interest. The method is useful for detecting specific nucleic acids in a sample with high specificity and sensitivity. The method also has an inherently low level of background signal. A preferred form of the method consists of a DNA ligation operation, an amplification operation, and a detection operation. The DNA ligation operation circularizes a specially designed nucleic acid probe molecule. This operation is dependent on hybridization of the probe to a target sequence and forms circular probe molecules in proportion to the amount of target sequence present in a sample. The amplification operation is rolling circle replication of the circularized probe. A single round of amplification using rolling circle replication results in a large amplification of the circularized probe sequences. Following rolling circle replication, the amplified probe sequences are detected and quantified using any of the conventional detection systems for nucleic acids such as detection of fluorescent labels, enzyme-linked detection systems, antibody-mediated label detection, and detection of radioactive labels. Because, the amplified product is directly proportional to the amount of target sequence present in a sample, quantitative measurements reliably represent the amount of a target sequence in a sample. Major advantages of this method are that the ligation step can be manipulated to obtain allelic discrimination, the DNA replication step is isothermal, and signals are strictly quantitative because the amplification reaction is linear and is catalyzed by a highly processive enzyme. In multiplex assays, the primer oligonucleotide used for the DNA polymerase reaction can be the same for all probes. Also described are modes of the method in which additional amplification is obtained using a cascade of strand displacement reactions.
REFERENCES:
patent: 2264423 (1941-12-01), Wingenroth
patent: 3687808 (1972-08-01), Merigan et al.
patent: 3921105 (1975-11-01), Brgetz
patent: 3983421 (1976-09-01), Yogore
patent: 4469863 (1984-09-01), Ts'o
patent: 4476301 (1984-10-01), Imbach et al.
patent: 4748111 (1988-05-01), Dattagupta et al.
patent: 4845205 (1989-07-01), Huynh Dinh et al.
patent: 4883750 (1989-11-01), Whiteley et al.
patent: 4965188 (1990-10-01), Bertram et al.
patent: 4981957 (1991-01-01), Lebleu et al.
patent: 4984957 (1991-01-01), Noguchi et al.
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
patent: 5001050 (1991-03-01), Blanco et al.
patent: 5023243 (1991-06-01), Tullis
patent: 5034506 (1991-07-01), Summerton et al.
patent: 5043272 (1991-08-01), Hartley
patent: 5118800 (1992-06-01), Smith et al.
patent: 5130238 (1992-07-01), Malek et al.
patent: 5130302 (1992-07-01), Spielvogel et al.
patent: 5134066 (1992-07-01), Rogers et al.
patent: 5166315 (1992-11-01), Summerton et al.
patent: 5175273 (1992-12-01), Bischofberger et al.
patent: 5177196 (1993-01-01), Meyer, Jr. et al.
patent: 5185444 (1993-02-01), Summerton et al.
patent: 5188897 (1993-02-01), Suhadolnik et al.
patent: 5198543 (1993-03-01), Blanco et al.
patent: 5214134 (1993-05-01), Weis et al.
patent: 5216141 (1993-06-01), Benner
patent: 5235033 (1993-08-01), Summerton et al.
patent: 5242794 (1993-09-01), Whiteley et al.
patent: 5264423 (1993-11-01), Cohen et al.
patent: 5264562 (1993-11-01), Matteucci
patent: 5264564 (1993-11-01), Matteucci
patent: 5264567 (1993-11-01), Numata et al.
patent: 5273638 (1993-12-01), Konrad et al.
patent: 5276019 (1994-01-01), Cohen et al.
patent: 5278302 (1994-01-01), Caruthers et al.
patent: 5286717 (1994-02-01), Cohen et al.
patent: 5319080 (1994-06-01), Leumann
patent: 5321131 (1994-06-01), Agrawal et al.
patent: 5328824 (1994-07-01), Ward et al.
patent: 5354668 (1994-10-01), Auerbach
patent: 5359044 (1994-10-01), Cook et al.
patent: 5367066 (1994-11-01), Urdea et al.
patent: 5367069 (1994-11-01), Beck et al.
patent: 5393878 (1995-02-01), Leumann
patent: 5399676 (1995-03-01), Froehler
patent: 5405938 (1995-04-01), Summerton et al.
patent: 5405939 (1995-04-01), Suhadolnik et al.
patent: 5409818 (1995-04-01), Davey et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5427930 (1995-06-01), Birkenmeyer et al.
patent: 5429807 (1995-07-01), Matson et al.
patent: 5432272 (1995-07-01), Benner
patent: 5434257 (1995-07-01), Matteucci et al.
patent: 5443986 (1995-08-01), Haughland et al.
patent: 5446137 (1995-08-01), Maag et al.
patent: 5451067 (1995-09-01), Pieper
patent: 5451203 (1995-09-01), Lamb
patent: 5453496 (1995-09-01), Caruthers et al.
patent: 5455166 (1995-10-01), Walker
patent: 5455233 (1995-10-01), Spielvogel et al.
patent: 5457187 (1995-10-01), Gmeiner et al.
patent: 5459255 (1995-10-01), Cook et al.
patent: 5466677 (1995-11-01), Baxter et al.
patent: 5466786 (1995-11-01), Buhr et al.
patent: 5470967 (1995-11-01), Huie et al.
patent: 5476427 (1995-12-01), Fujima
patent: 5476925 (1995-12-01), Letsinger et al.
patent: 5484908 (1996-01-01), Froehler et al.
patent: 5489677 (1996-02-01), Sanghvi et al.
patent: 5502177 (1996-03-01), Matteucci et al.
patent: 5510270 (1996-04-01), Fodor et al.
patent: 5514785 (1996-05-01), Van Ness et al.
patent: 5516134 (1996-05-01), Crawford et al.
patent: 5516663 (1996-05-01), Backman et al.
patent: 5519126 (1996-05-01), Hecht
patent: 5519134 (1996-05-01), Acevedo et al.
patent: 5521065 (1996-05-01), Whitley et al.
patent: 5523204 (1996-06-01), Singer et al.
patent: 5525711 (1996-06-01), Hawkins et al.
patent: 5536821 (1996-07-01), Agrawal et al.
patent: 5538871 (1996-07-01), Nuovo et al.
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5541306 (1996-07-01), Agrawal et al.
patent: 5541307 (1996-07-01), Cook et al.
patent: 5547843 (1996-08-01), Studier et al.
patent: 5550111 (1996-08-01), Suhadolnik et al.
patent: 5552540 (1996-09-01), Haralambidis
patent: 5556772 (1996-09-01), Sorge et al.
patent: 5561225 (1996-10-01), Maddry et al.
patent: 5563037 (1996-10-01), Sutherland et al.
patent: 5563253 (1996-10-01), Agrawal et al.
patent: 5563912 (1996-10-01), Yasunaga et al.
patent: 5567811 (1996-10-01), Misiura et al.
patent: 5571799 (1996-11-01), Tkachuk et al.
patent: 5576427 (1996-11-01), Cook et al.
patent: 5587361 (1996-12-01), Cook et al.
patent: 5587469 (1996-12-01), Cook et al.
patent: 5591609 (1997-01-01), Auerbach
patent: 5591722 (1997-01-01), Montgomery et al.
patent: 5594121 (1997-01-01), Froehler et al.
patent: 5596086 (1997-01-01), Matteucci et al.
patent: 5596091 (1997-01-01), Switzer
patent: 5597909 (1997-01-01), Urdea et al.
patent: 5599921 (1997-02-01), Sorge et al.
patent: 5602240 (1997-02-01), De Mesmaeker et al.
patent: 5608046 (1997-03-01), Cook et al.
patent: 5610289 (1997-03-01), Cook et al.
patent: 5610300 (1997-03-01), Altmann et al.
patent: 5614389 (1997-03-01), Auerbach
patent: 5614390 (1997-03-01), McCaslin et al.
patent: 5614617 (1997-03-01), Cook et al.
patent: 5618704 (1997-04-01), Sanghui et al.
patent: 5623070 (1997-04-01), Cook et al.
patent: 5625050 (1997-04-01), Beaton et al.
patent: 5627053 (1997-05-01), Usman et al.
patent: 5629158 (1997-05-01), Uhlen
patent: 5629179 (1997-05-01), Mierendorf et al.
patent: 5633360 (1997-05-01), Bischofberger et al.
patent: 5639873 (1997-06-01), Barascut et al.
patent: 5646265 (1997-07-01), McGee
patent: 5648245 (1997-07-01), Fire et al.
patent: 5658873 (1997-08-01), Bertsch-Frank et al.
patent: 5663312 (1997-09-01), Chaturvedula
patent: 5670633 (1997-09-01), Cook et al.
patent: 5677437 (1997-10-01), Teng et al.
patent: 5677439 (1997-10-01), Weis et al.
patent: 5681941 (1997-10-01), Cook et al.
patent: 5700920 (1997-12-01), Altmann et al.
patent: 5710028 (1998-01-01), Eyal et al.
patent: 5714320 (1998-02-01), Kool
patent: 5714331 (1998-02-01), Buchardt et al.
patent: 5719262 (1998-02-01), Buchardt et al.
patent: 5728526 (1998-03-01), George et al.
patent: 5733733 (1998-03-01), Auerbach
patent: 5766891 (1998-06-01), Shuman
patent: 5795714 (1998-08-01), Cantor et al.
patent: 5821084 (1998-10-01), Olmsted et al.
patent: 5854033 (1998-12-01), Lizardi
patent: 585405
Ballard Spahr LLP
Myers Carla
Yale University
LandOfFree
Rolling circle replication reporter systems does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rolling circle replication reporter systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rolling circle replication reporter systems will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4067834