Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-11-21
1998-12-29
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 911, 435 6, 536 231, 536 243, C12P 1934, C12Q 168, C07H 2102, C07H 2104
Patent
active
058540334
ABSTRACT:
Disclosed are compositions and a method for of amplifying nucleic acid sequences useful for detecting the presence of molecules of interest. The method is useful for detecting specific nucleic acids in a sample with high specificity and sensitivity. The method also has an inherently low level of background signal. A preferred form of the method consists of a DNA ligation operation, an amplification operation, and a detection operation. The DNA ligation operation circularizes a specially designed nucleic acid probe molecule. This operation is dependent on hybridization of the probe to a target sequence and forms circular probe molecules in proportion to the amount of target sequence present in a sample. The amplification operation is rolling circle replication of the circularized probe. A single round of amplification using rolling circle replication results in a large amplification of the circularized probe sequences. Following rolling circle replication, the amplified probe sequences are detected and quantified using any of the conventional detection systems for nucleic acids such as detection of fluorescent labels, enzyme-linked detection systems, antibody-mediated label detection, and detection of radioactive labels. Because, the amplified product is directly proportional to the amount of target sequence present in a sample, quantitative measurements reliably represent the amount of a target sequence in a sample. Major advantages of this method are that the ligation step can be manipulated to obtain allelic discrimination, the DNA replication step is isothermal, and signals are strictly quantitative because the amplification reaction is linear and is catalyzed by a highly processive enzyme. In multiplex assays, the primer oligonucleotide used for the DNA polymerase reaction can be the same for all probes. Also described are modes of the method in which additional amplification is obtained using a cascade of strand displacement reactions.
REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5001050 (1991-03-01), Blanco et al.
patent: 5198543 (1993-03-01), Blanco et al.
patent: 5409818 (1995-04-01), Davey et al.
patent: 5455166 (1995-10-01), Walker
Johnstone and Thorpe, "The Insoluble Derivative", Immounochemistry in Practice (Blackwell Scientific Publications, Oxford, England, 1987) pp. 209-216 and 241-242.
Landergren, "Molecular mechanics of nucleic acid sequence amplifications", Trends Genetics, 9:199-202 (1993).
McGraw et al., "Sequence-Dependant Oligonucleotide-Target Duplex Stabilities: Rules from Empirical Studies with a Set of twenty-Mers" Biotechniques 8:674-678 (1990).
Balanco ans Salas, "Characterization and purification of a phage .PI.29-encoded DNC polymeras required for the initation of replication", Proc. Natl. Acad. Sci. USA , 81: 5325-5329 (1984).
Blanco et al., "Highly Efficient DNA Synthesis by the Phage .PI.29 DNA Polymerase", Journal of Biological Chemistry ., 264(15): 8935-8940 (1989).
Blanco et al., "Terminal protein-primed DNA amplication", Proc. Natl. Acad. Sci. USA , 91: 12198-12202 (1994).
Boehmer and Lehman, "Herpes Simplex Virus Type 1 lCP8: Helix-Destabilizing Properties", Journal of Virology , 67(2): 711-715 (1993).
Broude et al., "Enhanced DNA sequencing by hrbridization", Proc. Natl. Acad Sci. USA , 91: 3072-3076 (1994).
Butler and Chamberlin, "Bacteriophage SP6-specific RNA Polymerase", Journal of Biological Chemistry , 257: 5772-578 (1982).
Chatteriee et al., "Cloning and overexpression of the gene encoding bacteriophage T5 DNA polmerase", Journal of Biological Chemistry , 257: 5772-5778 (1982).
Davanloo et al., "Clonong and expression of the gene for bacteriophage T7 RNA polymerase", Proc. Natl. Acad. Sci. USA , 81: 2035-2039 (1984).
Fire and Xu, "Rolling replication of short DNA circles", Proc. Natl. Acad. Sci. USA , 92: 4641-4645 (1995).
Gasparro et al., "Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: characterization of psoralen monoadduct and crosslink formation", Nucleic Acids Research , 22(14): 2845-2852 (1994).
Gunji et al., "Correlation Between the Serum Level of Hepatitis C Virus RNA and Disease Activities in Acute and Chronic Hepatitis C", Int. J. Cancer , 52(5): 726-730 (1992).
Guo et al. "Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports", Nucleic Acids Res ., 22(24): 5456-5645 (1994).
Gupta et al., "Ninth International Conference on AIDS/Fourth STD World Congress ", June 6-11, 1993, Berlin, Germany.
Hagiwara et al., "Quantitation of hepatitis C Virus RNA in Serum of Asymptomatic Blood Donors and Patients with Type C Chronic Liver Disease", Hepatology , 17(4): 545-550 (1993).
Hanvey et al., "Antisense and Antigene Properties of Peptide Nucleic Acids", Science, 258: 1481-1485 (1992).
Abravaya et al., "Detection of point mutations with a modified ligase chain reaction (Gap-LCR)", Nucleic Acids Res., 23(4): 675-682 (1995).
Alves and Carr, "Dot blot detection of point mutations with adjacently hybridising synthetic oligonucleotide probes", Nucleic Acids Res., 16(17): 8723 (1988).
Arnold et al., "Assay Formats Involving Acridinium-Ester-Labeled DNA Probes", Clin. Chem., 35(8): 1588-1594 (1989).
Barany, "Genetic disease detection and DNA amplication using clones thermosatable ligase", Proc. Natl. Acad. Sci. USA, 88: 189-193 (1991).
Bertina et al., "Mutation in blood coaqulation for V. associated with resistance to activated protein C", Nature, 369: 64-67 (1994).
Birkenmeyer and Mushahwar , "DNA probe amplification methods", Journal of Virological Methods, 35: 117-126 (1991).
Balanco and Salas, "Characterizations and purification of a phage .PI.29-encoded DNC polymerase required for the initiation of replication", Proc. Natl. Acad. Sci. USA, 81: 5325-5329 (1984).
Blanco et al., "Highly Efficient DNA Synthesis by the Phage .PI.29 DNA Polymerase", Journal of Biological Chemistry, 264(15): 8935-8940 (1989).
Blanco et al., "Terminal protein-primed DNA amplification", Proc. Natl. Acad Sci. USA, 91: 12198-12202 (1994).
Boehmer and Lehman, "Herpes Simplex Virus Type 1 ICP8: Helix-Destablizing Properties", Journal of Virology, 67(2): 711-715 (1993).
Broude et al., "Enhanced DNA sequencing by hybridization", Proc. Natl. Acad Sci. USA, 91: 3072-3076 (1994).
Butler and Chamberlin, "Bacteriophage SP6-specific RNA Polymerase", Journal of Biological Chemistry, 257: 5772-5778 (1982).
Chatterjee et al., "Cloning and overexpression of the gene encoding bacteriophage T5 DNA polymerase", Gene, 97: 13-19 (1991).
Davanloo et al., "Cloning and expression of the gene for bacteriophage T7 RNA polymerase", Proc. Natl. Acad. Sci. USA, 81: 2035-2039 (1984).
Fire and Xu, "Rolling replication of short DNA circles", Proc. Natl. Acad Sci. USA, 92: 4641-4645 (1995).
Gasparro et al., "Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: characterizations of psoralen monoadduct and crosslink formation" Nucleic Acids Research, 22(14): 2845-2852 (1994).
Gunji et al., "Correlation Between the Serum Level of Hepatitis C Virus RNA and Disease Activities in Acute and Chronic Hepatitis C", Intl. J. Cancer, 52(5): 726-730 (1992).
Guo et al., "Direct fluorescence analysis of genetic of genetic polymorphisms by hrbridization with oligonucleotide arrays on glass supports", Nucleic Acids Res., 22(24): 5456-5465 (1994).
Gupta et al., "Ninth International Conference on AIDS/Fourth STS World Congress", Jun.6-11, 1993, Berlin, Germany.
Hagiwara et al., "Quantitation of hepatitis C Virus RNA in Serum of Asymptomatic Blood Dodnors and Patients with Type C Chronic Liver Disease", Hepatology, 17(4): 545-550 (1993).
Hanvey et al., "Antisense and Antigene Properties of Peptide Nucleic Acids", Science, 258: 1481-1485 (1992).
Hata et al., "Structure of the Human Ornithine Transcarbamylase Gene", J. Biochem., 103: 302-308 (1988).
Hendrickson et al., "High sensitivity multianalyte immunoassay using covalent DNA-labeled antibodies and polymerase chain reaction", Nucleic Acids Res., 23(3): 522-529 (1995).
Holloway et al., "An exonuc
Whisenaut Ethan
Yale University
Zitomer Stephanie W.
LandOfFree
Rolling circle replication reporter systems does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rolling circle replication reporter systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rolling circle replication reporter systems will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1423543