Rolling circle replication of padlock probes

Details Rolling circle replication of padlock probes Rolling circle replication of padlock probes

2006-07-11

2006-07-11

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200

Reexamination Certificate

active

07074564

ABSTRACT:
Rolling circle replication of a padlock primer is inhibited when it is hybridised to a target nucleic acid that is long or circular. The invention provides methods of addressing this problem including cutting the target nucleic acid near or preferably at the site which hybridises with the padlock probe, whereby a 3′-end of the cut target nucleic acid acts as a primer for rolling circle replication of the padlock probe. Also included is a method for assaying for a polyepitopic target by the use of two affinity probes each carrying an oligonucleotide tag and of padlock probe for rolling circle replication in association with the two affinity probes.

REFERENCES:
patent: 5854033 (1998-12-01), Lizardi
patent: 6878515 (2005-04-01), Landegren
patent: 97/00446 (1997-01-01), None
patent: 9719193 (1997-05-01), None
Baner et al., Nucleic Acids Res. 26(22). 5073-5078, Nov. 15, 1998.

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