Roller bottle having pitted or cratered inner surface for cell g

Details Roller bottle having pitted or cratered inner surface for cell g Roller bottle having pitted or cratered inner surface for cell g

1998-02-27

2000-10-10

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

4352871, 4352881, 435325, 435394, 435395, 435410, C12M 300, C12N 100, C12N 500, C12N 502

Patent

active

061300802

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND OF THE INVENTION

The present invention relates to vessels having textured surfaces for growing animal cells, plant cells, micro-organisms and the like (`bio-substances`) and to methods for their production.
Many bio-substances can be propagated in vitro in containers such as so-called `roller bottles`, that is, cylindrical vessels, usually of glass or polystyrene, which are partially filled with a liquid medium comprising the bio-substances. The bottles are arranged to be rotated slowly about their longitudinal axes, promoting aeration, while the liquid medium provides nutrients to the bio-substances as they grow on the inner surfaces of the container.
Limitations in the efficiency of conventional procedures have hitherto been due to the relatively small surface area provided by the smooth interior of the container, as well as the fact that such surfaces do not provide optimum characteristics for the growth processes. It is an object of the present invention to eliminate or minimise these disadvantages.


SUMMARY OF THE INVENTION

Accordingly, in a first aspect of the invention, there is provided apparatus for use in a method of culturing micro-biological material, wherein said apparatus defines a rough or uneven growth surface arranged for contact with micro-biological material being cultured. An advantage of the inventive apparatus is that its use can allow cultured material to be grown at enhanced rates.
Preferably, the growth surface is rendered rough or uneven by surface features having a depth of 1 mm or less and, preferably, a depth of 0.1-0.5 mm. In preferred embodiments, the growth surface is pitted, or pockmarked with a plurality of craters or crater like depressions, preferably to depths of 1 mm or less and more preferably to depths of 0.1-0.5 mm. Preferably, the craters or crater like depressions measure less than 5, preferably less than 2, and more preferably less than 1 mm across. Most preferably, the crater or crater like depressions measure less than 0.5 mm across.
In further embodiments, the growth surface is micro-cupellated and it can be formed from an organic polymer. The polymer can be coated onto a supporting substrate and, preferably, is a silicone rubber. In an embodiment, the growth surface forms an inner surface of a container for retaining biological material being cultured.
In a second aspect, the invention provides a method of culturing a micro-biological material, comprising culturing said material using apparatus defining a growth surface, wherein said material is in contact with the growth surface and the growth surface is uneven or rough. Advantageously the inventive method allows biological material to be grown at an enhanced rate.
Preferably, the apparatus employed in the second aspect of the invention is in accordance with its first aspect.
In a third aspect, the invention provides a method of providing a substrate with a textured surface comprising the steps of forming a mobile surface studded with a granular material on the substrate, setting the mobile surface and removing the granular material to leave a textured surface on said substrate.
In a fourth aspect of the invention, there is provided an article having a textured surface that can be obtained by a method according to the invention in its third aspect. Preferably, the article is a tissue or cell culture vessel, such as a roller-bottle.
In all aspects of the invention the biological material preferably comprises tissue, cells or like matter, including viral particles or virions.
In the invention according to its fourth aspect, the mobile surface is preferably tacky prior to the setting step, so as to promote adherence of the granular material. In a preferred embodiment, a (preferably solvent-less) silicone paint, designed for polymerisation to form silicone rubber, is applied to the interior surface of a glass or polystyrene roller bottle. While the coating is still in liquid form, common salt particles are injected into the container, for example, under pressure, to adhere to the prepared silicone

REFERENCES:
patent: 3976547 (1976-08-01), McAleer et al.
patent: 4407954 (1983-10-01), Clyde
patent: 4689301 (1987-08-01), Adet et al.

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