Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1994-09-12
1996-06-18
Czaja, Donald E.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435240241, 435240242, 4352992, 4353042, 220 413, 220400, 220404, 220413, 220426, 220660, 220661, 220662, 220DIG14, C12M 300, C12M 124, C12N 500, B65D 9004
Patent
active
055277055
ABSTRACT:
A roller bottle for trans-membrane co-culture of cells includes an exterior receptacle with a longitudinal axis having a first chamber surrounded by a sidewall. The exterior receptacle has a first neck at one end having an opening therethrough providing fluid access to said first chamber. The roller bottle further includes an interior container with a longitudinal axis and a second chamber, the interior container being located coaxially within the exterior receptacle. The interior container has a second neck at one end providing fluid access to the second chamber. The exterior receptacle is formed from a material that is substantially impermeable to gas and liquid and is sealed in a substantially fluid tight fashion forming the first chamber that has fluid access by the first neck. At least a portion of the interior container is formed from a microporous material. The roller bottle of the invention enables culturing one cell type in the presence of another cell type for the study of interactions between the cells while maintaining a physical separation between the cell types providing a scale up in cell population size from well type inserts. The microporous membrane allows free interchange of media soluble cellular products between the cell types, but maintains physical separation.
REFERENCES:
patent: 3941661 (1976-03-01), Noteboom
patent: 4238568 (1980-12-01), Lynn
patent: 4308351 (1981-12-01), Leighton et al.
patent: 4337104 (1982-06-01), Lynn
patent: 4350575 (1982-09-01), Porta et al.
patent: 4830970 (1989-05-01), Madaus et al.
patent: 4912058 (1990-03-01), Mussi et al.
patent: 5139953 (1992-08-01), Honda et al.
patent: 5260210 (1993-11-01), Rubin et al.
Miller et al, Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier, J. Tiss. Cult. Meth. 14:217-224, 1992.
Madara et al, A Simple Approach to Measurement of Electrical Parameters of Cultured Epithelial Monolayers: Use in Assessing Neutrophil-epithelial Interactions, J. Tiss. Cult. Meth. 14:209-216, 1992.
Mangum et al, Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins, In Vitro Cell. Dev. Biol. 26:1135-1143, Dec. 1990.
C. Grobstein, Trans-Filter Induction of Tubules in Mouse Metanephrogenic Mesenchyme, Experimental Cell Research, 10, 424-440.
Frontiers in biology: Development, Looking to Development's Future, Science, vol. 266, 28 Oct. 1994, 561-570.
Gray Harry E.
Mussi Edward F.
Becton Dickinson and Company
Czaja Donald E.
Dawson Arthur D.
Elkin Jane Williams
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