RNA transformation vector

Details RNA transformation vector RNA transformation vector

1995-06-05

1998-12-08

Guzo, David

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 435419, 536 2372, 536 241, 536 245, C12N 1533, C12N 510, C12N 1582, C07H 2102

Patent

active

058467955

ABSTRACT:
A +strand RNA viral transformation of host organisms with foreign RNA, and expression of said foreign RNA. The foreign RNA is inserted into an infective RNA viral segment containing replication elements, and allowed to infect the host organism. The invention is exemplified utiliing brome mosaic RNA modified to contain a gene coding for chloramphenicol acetyl transferase (CAT) in the transformation of barley protoplasts.

REFERENCES:
patent: 5500360 (1996-03-01), Ahlquist et al.
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Hasseloff, J. et al. (1984) Proc. Natl. Acad. Sci. USA 81:4358.
Ahlquist, P. et al. (1985) J. Virol. 53:536.
Ahlquist, P. et al. (1981) J. Mol. Biol. 153:23.
Ahlquist, P. et al. (1984) J. Mol. Biol. 172:369.
Ahlquist, P. and Janda, M. (1984) Mol. Cell Biol. 4:2876.
Siegel, A. (1985) Plant Mol. Biol. 4:327-329.
Van Vloten-Doting et al. (1985) Plant Mol. Biol. 4:323-326.
French, R. and Ahlquist, P. (1987) J. Virol. 61:1457-1465.
Ahlquist, P. et al. (1987) Adv. Virus Res. 32:215-242.
Ahlquist, P. and French, R. (1988) in RNA GEnetics Book 2: RNA Variability, Domingo et al. (eds.), CRC Press, Orlando, Florida.
Strauss et al. 1994. Microbiological Reviews 58: 491-562.

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