RNA separation

Details RNA separation RNA separation

1996-09-04

1998-07-07

Kight, John

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 251, 536 254, 536 2541, C07H 2102

Patent

active

057770999

ABSTRACT:
An improved process for the separation of RNA from a liquid sample of biological origin containing RNA comprises (a) intimately contacting the sample with a two-phase composition wherein the upper phase is predominately phenol and the lower phase is an aqueous solution comprising at least one water-soluble guanidinium salt, a buffer and urea, the water content of the aqueous phase being relatively low, (b) adding a relatively dense water-insoluble solvent and (c) recovering RNA from the resulting aqueous phase.

REFERENCES:
patent: 3389133 (1968-06-01), Gutcho
patent: 4830969 (1989-05-01), Holmes
patent: 4843155 (1989-06-01), Chomczynski
patent: 5130423 (1992-07-01), Van Ness et al.
patent: 5234809 (1993-08-01), Boom et al.
patent: 5300635 (1994-04-01), Macfarlane
patent: 5346994 (1994-09-01), Chomczynski
patent: 5393672 (1995-02-01), Van Ness et al.
patent: 5422241 (1995-06-01), Goldrick et al.
Chomczynski, "A Reagent for the Single-Step Simultaneous Isolation of RNA, DNA and Protein from Cells and Tissue Samples," Biotechniques, 15(3), 532-537 (1993).
Chomczynski et al., "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction," Analytical Biochemistry, 162(1), 156-159 (1987).
Siebert et al., "Modified Acid Guanidinium Thiocyanate-Phenol-Chloroform RNA Extraction Method Which Greatly Reduced DNA Contamination," Nucleic Acids Research, 21(8), 2019-2020 (1993).
R. A. Cox, "The Use of Guanidinium Chloride in the Isolation of Nucleic Acids," Ch. 103a in Method in Enzymology, vol. XII, Nucleic Acids, Part B, Grossman et al. eds., Academic Press, New York, NY, 1968, pp. 102-129.
Biotecx Bulletin No. 27, "Ultraspec RNA Isolation System," Biotecx Laboratories, Houston, TX, Feb., 1993.
Biotecx Bulletin No. 28, "Ultraspec-II RNA Isolation System," Biotecx Laboratories, Houston, TX, Oct., 1993.
Chirgwin et al., "Isolation of Biologically Active Ribonucleic Acid From Sources Enriched in Ribonuclease," Biochemistry, 18(24), 5294-5299 (1979).
Stedman's Medical Dictionary, 25th Ed., Williams and Wilkins, Baltimore, MD, 1990, see p. 283, col. 2.

Affiliated with

Also associated with

Rating

RNA separation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with RNA separation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and RNA separation will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1207322