Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1986-12-03
1991-01-22
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351723, 435193, 435194, 536 27, 935 3, 935 14, 502167, C12D 1934, C12N 1500, C12N 910, C12N 912, C07H 1512, B01J 3100
Patent
active
049870718
ABSTRACT:
RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.
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The invention was made in part with government funds under Grant GM 28039 from the National Institutes of Health. Therefore, the U.S. Government has certain rights in the invention.
Been Michael D.
Cech Thomas R.
Zaug Arthur J.
Lebovitz Richard M.
Teskin Robin L.
University Patents Inc.
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