RNA ribozyme polymerases, dephosphorylases, restriction...

Details RNA ribozyme polymerases, dephosphorylases, restriction... RNA ribozyme polymerases, dephosphorylases, restriction...

2000-10-10

2004-02-24

Brusca, John S. (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091310, C536S023200

Reexamination Certificate

active

06696250

ABSTRACT:

This invention concerns compositions of RNA functioning as an RNA enzyme, i.e. a ribozyme in several capacities: dephosphorylase (acid phosphatase and transphosphorylase), ribonucleotidyl transferase (polymnerase activity) and sequence-specific endoribonuclease activities.
SUMMARY
It is found that purified ribonucleic acid (RNA) can serve as an enzyme acting on other RNA molecules in vitro (ribozyme) as a: 1) dephosphorylating enzyme catalyzing the removal of 3′ terminal phosphate of RNA in a sequence-specific manner, 2) RNA polymerase (nucleotidyltransferase) catalyzing the conversion of oligoribonucleotides to polyribonucleotides, 3) sequence specific endoribonuclease. (This latter activity is also referred to as RNA restriction endonuclease or endoribonuclease activity.)


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