RNA probe for detecting c-fes mRNA

Details RNA probe for detecting c-fes mRNA RNA probe for detecting c-fes mRNA

1999-03-04

2001-05-15

Whisenant, Ethan (Department: 1658)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

06232069

ABSTRACT:

The present invention is related generally to diagnostic tests. More particularly, the present invention is related to an RNA probe for detecting the presence of c-fes mRNA in biological samples, such as human cell and tissue RNA preparations.
Expression of the c-fes oncogene is known to play a certain functional role in myelopoiesis in hematopoietic cells (Smithgall et al, 1988,
J. Biol. Chem.
263, 15050-15055; Greer et al, 1988,
Mol. Cell. Biol.,
8, 578-587). However, heretofore direct evidence was lacking to prove that the expression of human c-fes gene induced myeloid differentiation in cells. Furthermore, a specific and sensitive assay to measure the level of c-fes mRNA in human cells and tissues was also heretofore not available.
SUMMARY OF THE INVENTION
It is, therefore, an object of the present invention to provide a kit for the detection of c-fes mRNA in biological samples such as human cell and tissue RNA preparations.
It is a further object of the present invention to provide an RNA probe for detecting the presence of c-fes mRNA in vitro or in situ.
It is another object of the present invention to provide a recombinant plasmid comprising exon 2 of the human c-fes genomic sequence for the expression of the transcription product of the c-fes oncogene in a suitable expression vector.
Other objects and advantages will become evident from the following detailed description of the invention.


REFERENCES:
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patent: 5968825 (1999-10-01), Ferrari et al.
Feldman et al., Oncogene Research 1(4) : 441-458 (Sep.-Oct. 1987).*
Matthews et al., Analytical Strategies for the Use of DNA Probes. Analytical Biochemistry 169 : 1-25 (1988).*
The Stragene Catalog p. 39 (1988).*
Avers,Molecular Cell Biology, Addison-Wesley Publishing Company (1986).
Promega Catalog and Applications Guide, p. 8 and 18 (1988).
Roebroek et al., The EMBO Journal 4(11)2897-2903 (1985).
Melton et al., Nucleic Acids Research, 12(18):7035-7056 (1994).
DeBold et al., Endocrinology 66(6):2648-2657 (1988).
Watson et al., Molecular Biology of the Gene, Benjmain/Cummings Publishing Co., Inc. (1987).

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