RNA polymerase II holoenzyme from Saccharomyces cerevisiae

Details RNA polymerase II holoenzyme from Saccharomyces cerevisiae RNA polymerase II holoenzyme from Saccharomyces cerevisiae

1994-03-25

1999-07-13

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

530350, 530371, 536 2374, 536 232, 536 241, C12N 912, C12N 1554, C12N 1531

Patent

active

059225858

ABSTRACT:
An RNA polymerase II holoenzyme that contains, in addition to RNA polymerase, a subset of the general transcription factors together with nine SRB proteins is described. This holoenzyme will selectively initiate transcription in vitro when supplemented with TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.

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