RNA polymerase gene, microorganism having said gene and the prod

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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536 27, 4351723, 435 691, 43525233, C12N 1500, C12N 912, C12N 1554, C12N 1570

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active

050266454

ABSTRACT:
SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.

REFERENCES:
Kassavetis et al., Journal of Billogical Chemistry (1982), vol. 257, No. 10, pp. 5779-5786.
Studier et al., U.S. 6595016 (U.S. Department of Energy) published for exploitation, 8 Oct., 1985.
Kotani et al., Nucleic Acids Research (1987), vol. 15, No. 6, pp. 2653-2664.
Davanloo et al., Proceedings of the National Academy of Sciences (USA) (1984), vol. 81, pp. 2035-2039.
Butler, Journal of Biological Chemistry, vol. 257, No. 10, pp. 5772-5778 (1982).

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