Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2005-03-15
2005-03-15
Hutson, Richard (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S193000, C435S091100, C435S440000, C530S350000, C530S358000, C536S023100, C536S023200
Reexamination Certificate
active
06867027
ABSTRACT:
Disclosed are RNA polymerases consisting of a wild type RNA polymerase provided that at least one of amino acids in the wild type RNA polymerase has been modified to enhance its ability for incorporating 3′-deoxyribonucleotides and derivatives thereof in comparison with the corresponding wild type RNA polymerases. Specifically, disclosed are, for example, the RNA polymerases wherein at least one amino acid present in a nucleotide binding sites of the wild type RNA polymerases such as phenylalanine has been replaced with tyrosine. The RNA polymerases of the present invention are a RNA polymerase which exhibits little or no bias for incorporation between ribonucleotides and 3′-deoxyribonucleotide as well as among ribonucleotides having different base groups and among deoxyribonucleotides having. different base groups.
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Hayashizaki Yoshihide
Watahiki Masanori
Burns Doane Swecker & Mathis L.L.P.
Hutson Richard
Nippon Gene Co., Ltd.
Nippon Genetech Co., Ltd.
The Institute of Physical and Chemical Research
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