RNA interference mediating small RNA molecules

Details RNA interference mediating small RNA molecules RNA interference mediating small RNA molecules

2006-07-18

2006-07-18

Wang, Andrew (Department: 1635)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C514S04400A, C536S023100, C536S024500

Reexamination Certificate

active

07078196

ABSTRACT:
Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using aDrosophilain vitro system, we demonstrate that 19–23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3′ ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.

REFERENCES:
patent: 5578716 (1996-11-01), Szyf et al.
patent: 5580859 (1996-12-01), Felgner et al.
patent: 5770580 (1998-06-01), Ledley et al.
patent: 5908779 (1999-06-01), Carmichael et al.
patent: 5972704 (1999-10-01), Draper et al.
patent: 5998203 (1999-12-01), Matulic-Adamic et al.
patent: 6225290 (2001-05-01), German et al.
patent: 6475726 (2002-11-01), Tally et al.
patent: 6531647 (2003-03-01), Baulcombe et al.
patent: 2002/0086356 (2002-07-01), Tuschl et al.
patent: 2002/0114784 (2002-08-01), Li et al.
patent: 2002/0132257 (2002-09-01), Giordano et al.
patent: 2002/0137210 (2002-09-01), Churikov
patent: 2002/0160393 (2002-10-01), Symonds et al.
patent: 2003/0068301 (2003-04-01), Draper et al.
patent: 2003/0108923 (2003-06-01), Tuschl et al.
patent: 2003/0140362 (2003-07-01), Macejak et al.
patent: 2003/0148985 (2003-08-01), Morrissey et al.
patent: 2003/0171311 (2003-09-01), Blatt et al.
patent: 2003/0190654 (2003-10-01), Heidenreich et al.
patent: 2003/0206887 (2003-11-01), Morrissey et al.
patent: 2004/0038921 (2004-02-01), Kreutzer et al.
patent: 2004/0053875 (2004-03-01), Kreutzer et al.
patent: 2004/0054156 (2004-03-01), Draper et al.
patent: 2004/0259248 (2004-12-01), Tuschl et al.
patent: 2 359 180 (2001-07-01), None
patent: 0 983 370 (2003-09-01), None
patent: 2 349 885 (2000-11-01), None
patent: 2 362 885 (2001-12-01), None
patent: 2 370 275 (2002-06-01), None
patent: WO 94/01550 (1994-01-01), None
patent: WO 97/11170 (1997-03-01), None
patent: WO 99/32619 (1999-07-01), None
patent: WO 99/49029 (1999-09-01), None
patent: WO 99/53050 (1999-10-01), None
patent: WO 99/61631 (1999-12-01), None
patent: WO 00/01846 (2000-01-01), None
patent: WO 00/32619 (2000-06-01), None
patent: 00/44895 (2000-08-01), None
patent: WO 00/44895 (2000-08-01), None
patent: WO 00/44895 (2000-08-01), None
patent: WO 00/63364 (2000-10-01), None
patent: WO 01/75164 (2001-10-01), None
patent: WO 01/75164 (2001-10-01), None
patent: WO 01/92513 (2001-12-01), None
patent: WO 02/44321 (2002-06-01), None
patent: WO 02/055692 (2002-07-01), None
patent: WO 02/059300 (2002-08-01), None
patent: WO 03/029459 (2003-04-01), None
patent: WO 03/033700 (2003-04-01), None
patent: WO 03/062394 (2003-07-01), None
patent: WO 03/099298 (2003-12-01), None
Parrish et al., Functional Anatomy of a dsRNA Trigger: Differential Requirement for the Two Trigger Strands in RNA Interference, Nov. 2000, Molecular Cell, vol. 6, pp. 1077-1087.
Alfonzo et al., The mechanism of U insertion/deletion RNA editing in kinetoplastid mitochondria, 1997, Nucleic Acids Research, vol. 25, No. 19, pp. 3751-3759.
Elbashir et al., Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells, 2001, Nature, vol. 411, pp. 494-498.
Elbashir et al., RNA interference is mediated by 21- and 22- nucleotide RNAs, 2001, Genes & Development, vol. 15, pp. 188-200.
Fire, A., et al., “Potent and Specific Genetic Interference By Double-Stranded RNA inCaenorhabditis elegans,” Nature 391:806-811 (Feb. 19, 1998).
Tuschl, T., et al., “Targeted mRNA Degradation By Double-Stranded RNA In Vitro,”Genes&Development 13:3191-3197 (1999).
Kennerdell, J.R. and Carthew, R.W., “Use of dsRNA-Mediated Genetic Interference to Demonstrate thatfrizzledandfrizzled 2Act in the Wingless Pathway,”Cell 95:1017-1026 (Dec. 23, 1998).
Montgomery, M.K. and Fire, A., “Double-Stranded RNA as a Mediator in Sequence-Specific Genetic Silencing and Co-suppression,”TIG 14(7):255-258 (Jul. 1998).
Bass B. L., “Double-Stranded RNA as a Template for Gene Silencing”Cell, Apr. 28, 2000, vol. 101, No. 3, pp. 235-238, XP-002194756.
Elbashir S. et al., “RNA Interference is Mediated by 21-and 22-Nucleotide RNAs”Genes and Development, Cold Spring Harbor Laboratory Press, New York, US, Jan. 15, 2001, vol. 15, No. 2, pp. 188-200, XP-002204651.
Hamilton A. J. et al., “A Species of Small Antisense RNA in Post-Transcriptional Gene Silencing in Plants”Science, American Association for the Advancement of Science, US, Oct. 29, 1999, vol. 286, No. 5441, pp. 950-952, XP-002149064.
Hammond S. M. et al., “An RNA-Directed Nuclease Mediates Post-Transcriptional Gene Silencing in Drosophilia Cells”Nature, Macmillan Journals LTD. London, GB, Mar. 16, 2000, vol. 404, No. 6775, pp. 293-296, XP-002183123.
Tuschl T. et al., “Targeted mRNA Degradation by Double-Stranded RNA in Vitro”Genes and Development, Cold Spring Harbor Laboratory Press, New York, US, Dec. 15, 1999, vol. 13, No. 24, pp. 3191-3197, XP-002183118.
Vinayak R. et al., “Chemical Synthesis of RNA Using Fast Oligonucleotide Deprotection Chemistry” Database Biosis Online!, Biosciences Information Service, Philadelphia, PA, US: 1992, Database Accession No. PREV199294000583, XP-002247596.
Yang D. et al., “Evidence That Processed Small dsRNAs May Mediated Sequence-Specific mRNA Degradation During RNAi in Drosophila Embryos”Current Biology, Sep. 19, 2000, vol. 10, No. 19, pp. 1191-1200, XP-001037751.
Zamore P. D. et al., “RNAi Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide Intervals”Cell, Cell Press, Cambridge, MA, US, Mar. 31, 2000, vol. 101, No. 1, pp. 25-33, XP-002208683.
Ngo, H., et al., “Double-Stranded RNA Induces mRNA Degradation inTrypanosoma brucei,” Proc. Natl. Acad. Sci. USA 95:14687-14692 (Dec. 1998).
Lohmann, J.U., et al., “Silencing of Developmental Genes inHydra,” Developmental Biology 214:211-214 (1999).
Clemens, M.J. and Williams, B.R.G., “Inhibition of Cell-Free Protein Synthesis by pppA2′p5′A2′p5′A: a Novel Oligonucleotide Synthesized by Interferon Treated L Cell Extracts,”Cell 13:565-572 (Mar. 1978).
Williams, B.R.G., et al., “The Respective Roles of the Protein Kinase and pppA2′p5′A2′p5′A-activated Endonuclease in the Inhibition of Protein Synthesis By Double-Stranded RNA in Rabbit Reticulocyte Lysates,”Nucleic Acids Research 6(4): 1335-1350 (Apr. 1979).
Zhou, A., et al., “Expression Cloning of 2-5A-Dependent RNAase: A Uniquely Regulated Mediator of Interferon Action,”Cell 72:753-765 (Mar. 12, 1993).
Zhou, A., et al., “Interferon Action in Triply Deficient Mice Reveals the Existence of Alternative Antiviral Pathways,”Virology 258:435-440 (1999).
Wianny, F. and Zernicka-Goetz, M., “Specific Interference With Gene Function By Double-Stranded RNA In Early Mouse Development,”Nature Cell Biol. 2:70-75 (Feb. 2000).
Gebauer, F., et al., “Translation Control of Dosage CompensationDrosophilaby Sex-lethal: Cooperative Silencing via the 5′ and 3′ UTRs ofmsl-2mRNA is Independent of the Poly(A) Tail,”The EMBO Journal 18(21):6146-6154 (1999).
Hamilton, A. J. and Baulcombe, D. C., “A Species of Small Antisense RNA in Posttrancriptional Gene Silencing in Plants,”Science 286:950-952 (Oct. 1999).
Wagner, R. W. and Sun, L., Double-stranded RNA Poses Puzzle,Nature 391:744-745 (Feb. 1998).
Sharp, P. A., “RNAi and Double-strand RNA,”Genes&Development 13(2):139-140 (Jan. 15, 1999).
Bass, B. L., “RNA Editing and Hypermutation by Adenosine Deamina

Affiliated with

Also associated with

Rating

RNA interference mediating small RNA molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with RNA interference mediating small RNA molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and RNA interference mediating small RNA molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3586783