RNA containing coenzymes, biotin, or fluorophores, and...

Details RNA containing coenzymes, biotin, or fluorophores, and... RNA containing coenzymes, biotin, or fluorophores, and...

2008-01-29

2008-01-29

Kaushal, Sumesh (Department: 1633)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C536S023100, C536S024100, C536S024300, C536S024500, C435S091300, C435S091500, C435S183000, C435S320100

Reexamination Certificate

active

10250029

ABSTRACT:
Materials and methods for incorporating adenosine derivatives into the 5′ end of transcribed RNA are disclosed. Adenosine derivatives include naturally occurring compounds such as Coenzyme A, NAD, and FAD, as well as various non-naturally occurring compounds. The derivatives can be used to impart desirable properties to the RNA such as fluorescence, the ability to bind to receptors or ligands, and improved catalytic activity. The transcribed RNAs can be used in a variety of applications including nucleic acid detection, designed or random generation of catalytic RNAs, antisense applications, and in the study of RNA structure and function.

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“Efficient Incorporation of CoA, NAD and FAD into RNA by in vitro transcription;” Faqing Huang; Nucleic Acids Research, 2003, vol. 31, Oxford University Press 2003.
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Coleman TM, Wang G, Huang F (2004).Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 ø2.5 promoter. Nucleic Acids Research. 32(1)e14.

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